Detailed information of TA system TA213855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5356..5936
• Replicon: plasmid pKP18-1-2
• Accession: NZ_CP082002
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K6149_RS27865
• Protein ID: WP_071177730.1
• Coordinates: 5356..5670 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K6149_RS27870
• Protein ID: WP_000093040.1
• Coordinates: 5658..5936 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS27840	1300..3264	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
K6149_RS27845	3264..3995	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
K6149_RS27850	4002..4532	+	531	WP_071177729.1	hypothetical protein	-
K6149_RS27855	4559..4738	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K6149_RS27860	4764..5192	-	429	WP_001140599.1	hypothetical protein	-
K6149_RS27865	5356..5670	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6149_RS27870	5658..5936	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K6149_RS29090	6111..6476	-	366	WP_072354022.1	TonB family protein	-
K6149_RS27880	6473..6844	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
K6149_RS27885	7118..7363	-	246	WP_032440458.1	hypothetical protein	-
K6149_RS27890	7690..9375	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
K6149_RS27895	9385..9642	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
K6149_RS27900	9727..9876	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10049	10049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T213855 WP_071177730.1 NZ_CP082002:c5670-5356 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T213855 NZ_CP109890:1157558-1157854 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT213855 WP_000093040.1 NZ_CP082002:c5936-5658 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT213855 NZ_CP109890:1157858-1158163 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1