Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5559092..5559717 Replicon chromosome
Accession NZ_CP082001
Organism Klebsiella pneumoniae strain KP18-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6149_RS27475 Protein ID WP_002882817.1
Coordinates 5559092..5559475 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6149_RS27480 Protein ID WP_004150355.1
Coordinates 5559475..5559717 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6149_RS27460 5556458..5557360 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6149_RS27465 5557357..5557992 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6149_RS27470 5557989..5558918 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6149_RS27475 5559092..5559475 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6149_RS27480 5559475..5559717 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6149_RS27485 5559922..5560839 + 918 WP_002882812.1 alpha/beta hydrolase -
K6149_RS27490 5560853..5561794 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6149_RS27495 5561839..5562276 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6149_RS27500 5562273..5563133 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6149_RS27505 5563127..5563726 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213854 WP_002882817.1 NZ_CP082001:c5559475-5559092 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213854 NZ_CP109890:457961-458263 [Xylella fastidiosa subsp. fastidiosa]
ATGGAGAAAGGCACATCTCACTATAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTCGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213854 WP_004150355.1 NZ_CP082001:c5559717-5559475 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213854 NZ_CP109890:458266-458667 [Xylella fastidiosa subsp. fastidiosa]
ATGAGATGTCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTACAAGAGCGAAACTAC
TAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCTTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCTTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGGTACGCCGGAAG
CTTGATCTCGACCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAACGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTCCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References