Detailed information of TA system TA213852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5138217..5138887
• Replicon: chromosome
• Accession: NZ_CP082001
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6149_RS25445
• Protein ID: WP_004213072.1
• Coordinates: 5138217..5138660 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6149_RS25450
• Protein ID: WP_004213073.1
• Coordinates: 5138657..5138887 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS25410	5133638..5133904	+	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-
K6149_RS25415	5133967..5134458	+	492	WP_011251327.1	DM13 domain-containing protein	-
K6149_RS25420	5134507..5135427	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6149_RS25425	5135518..5135921	+	404	Protein_4994	GAF domain-containing protein	-
K6149_RS25430	5136439..5137073	-	635	Protein_4995	mucoid phenotype regulator RmpA2	-
K6149_RS25435	5137490..5137794	+	305	Protein_4996	transposase	-
K6149_RS29085	5137817..5138068	-	252	WP_186987481.1	hypothetical protein	-
K6149_RS25445	5138217..5138660	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6149_RS25450	5138657..5138887	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6149_RS25455	5139495..5140628	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6149_RS25460	5140644..5140937	+	294	WP_004213076.1	hypothetical protein	-
K6149_RS25465	5140927..5141133	-	207	WP_004213077.1	hypothetical protein	-
K6149_RS25470	5141485..5141775	+	291	WP_004213078.1	hypothetical protein	-
K6149_RS25475	5141765..5142664	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187	iucA / iucB / iucC / iucD / iutA / rmpA	5044155..5240089	195934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T213852 WP_004213072.1 NZ_CP082001:c5138660-5138217 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T213852 NZ_CP109890:457306-457587 [Xylella fastidiosa subsp. fastidiosa]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT213852 WP_004213073.1 NZ_CP082001:c5138887-5138657 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213852 NZ_CP109890:457598-457873 [Xylella fastidiosa subsp. fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620