Detailed information of TA system TA213851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5061249..5061775
• Replicon: chromosome
• Accession: NZ_CP082001
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: K6149_RS25050
• Protein ID: WP_000323025.1
• Coordinates: 5061488..5061775 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: K6149_RS25045
• Protein ID: WP_000534858.1
• Coordinates: 5061249..5061488 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS25020	5056311..5057015	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6149_RS25025	5057247..5058587	+	1341	WP_000589001.1	ISNCY family transposase	-
K6149_RS25030	5059008..5059319	+	312	Protein_4914	transposase	-
K6149_RS25040	5060663..5060986	+	324	WP_224948387.1	cation transporter dimerization domain-containing protein	-
K6149_RS29070	5061120..5061224	-	105	Protein_4917	hypothetical protein	-
K6149_RS25045	5061249..5061488	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
K6149_RS25050	5061488..5061775	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
K6149_RS25055	5061847..5062005	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
K6149_RS25060	5062608..5063582	+	975	WP_004181899.1	hypothetical protein	-
K6149_RS25065	5063780..5064157	-	378	WP_004181901.1	hypothetical protein	-
K6149_RS25070	5064847..5065914	+	1068	WP_004181903.1	hypothetical protein	-
K6149_RS25075	5066070..5066417	+	348	WP_004181904.1	hypothetical protein	-
K6149_RS25080	5066496..5066729	+	234	WP_004181905.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187	iucA / iucB / iucC / iucD / iutA / rmpA	5044155..5240089	195934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T213851 WP_000323025.1 NZ_CP082001:5061488-5061775 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T213851 NZ_CP109889:c25702-25277 [Xylella fastidiosa subsp. fastidiosa]
ATGATTGTCCTCGACACGAACGTCATTTCCGAGCTGTGGAAGATCGAACCGCATCCCAACGTACTTGCGTGGATAGACGC
GCAGGCCATTGAAACGCTCTATCTGTCCGCCATCACCGTGGCCGAGCTGCGCTATGGTTTTGCGACGATGCCAGAGGGCA
AGCGCCGTACGGTCTTCCAGGAGCGGCTAGCGAACGAGGTTTTGCCGAATTTCGCGGGTCGCGTGCTTCCGTTCGATCTG
GATGCTTCCCAGGCCTATGCCGATCTCATGGCACGTGCGAGGGCAGAGGGCAAGGCCATCGGCAAGGCAGACGGTTACAT
CGCCGCCACAGCCGCCGCTCACGGTTTGATGGTCGCAACGCGCGACACCAGCCCTTTCCAAGCTGCTGGCCTGATTGTTA
TCGACCCTATTGGTCCTTCCCACTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT213851 WP_000534858.1 NZ_CP082001:5061249-5061488 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT213851 NZ_CP109889:c25956-25699 [Xylella fastidiosa subsp. fastidiosa]
ATGGCAAACGTGCATGTAAGAAATCTACCTGATGCTGTGCATCGCGCGCTTCGCGTGCGTGCCGCTCATAATGGCCGTAG
TACCGAGGCAGAAATCCGCGAAATCTTGGAGGTCGCTGTGCGACCGCCCGAGCGCGTCAAGCTCGGTTCCTTGCTGGCTT
CCATCGCTCGCGAGGCGGGCGGCCTGACCGATGCCGAGGTTGAACACTTCAATCAGCTCCGCGACAAGACGCCGACTGAG
ACGATGAGGTTTGAATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8