Detailed information of TA system TA213835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 328450..329036
• Replicon: chromosome
• Accession: NZ_CP082001
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: K6149_RS01575
• Protein ID: WP_002920800.1
• Coordinates: 328668..329036 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: K6149_RS01570
• Protein ID: WP_002920802.1
• Coordinates: 328450..328671 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS01550	324607..325533	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
K6149_RS01555	325530..326807	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
K6149_RS01560	326804..327571	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
K6149_RS01565	327573..328286	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
K6149_RS01570	328450..328671	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K6149_RS01575	328668..329036	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K6149_RS01580	329309..330625	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
K6149_RS01585	330732..331619	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
K6149_RS01590	331616..332461	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
K6149_RS01595	332463..333533	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	323436..334270	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T213835 WP_002920800.1 NZ_CP082001:328668-329036 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T213835 NZ_CP109875:104218-104634 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT213835 WP_002920802.1 NZ_CP082001:328450-328671 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT213835 NZ_CP109875:103991-104221 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4