Detailed information of TA system TA213834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 212..686
• Replicon: plasmid pKP18-1-3
• Accession: NZ_CP082000
• Organism: Klebsiella pneumoniae strain KP18-1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K6149_RS00010
• Protein ID: WP_004197762.1
• Coordinates: 212..478 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K6149_RS00015
• Protein ID: WP_000879771.1
• Coordinates: 462..686 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6149_RS00010	212..478	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6149_RS00015	462..686	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K6149_RS29145	849..977	+	129	WP_000523570.1	hypothetical protein	-
K6149_RS00020	1353..1565	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K6149_RS00025	1572..2081	-	510	WP_032433972.1	MbeB family mobilization protein	-
K6149_RS29150	2116..2550	-	435	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
K6149_RS00035	2759..3103	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
K6149_RS00040	3093..3269	-	177	WP_004197757.1	hypothetical protein	-
K6149_RS00045	4425..4751	+	327	WP_050516389.1	hypothetical protein	-
K6149_RS00050	4777..5322	+	546	WP_023317533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T213834 WP_004197762.1 NZ_CP082000:c478-212 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T213834 NZ_CP109875:c99807-99502 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT213834 WP_000879771.1 NZ_CP082000:c686-462 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT213834 NZ_CP109875:c100027-99809 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5