Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 1536488..1537103 Replicon plasmid pZ25
Accession NZ_CP081988
Organism Shinella oryzae strain Z-25

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag K6301_RS23355 Protein ID WP_247221215.1
Coordinates 1536807..1537103 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag K6301_RS23350 Protein ID WP_247221214.1
Coordinates 1536488..1536802 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6301_RS23330 (K6301_23290) 1532020..1534332 - 2313 WP_247221210.1 xanthine dehydrogenase family protein molybdopterin-binding subunit -
K6301_RS23335 (K6301_23295) 1534329..1535315 - 987 WP_247221211.1 xanthine dehydrogenase family protein subunit M -
K6301_RS23340 (K6301_23300) 1535312..1535821 - 510 WP_247221212.1 2Fe-2S iron-sulfur cluster-binding protein -
K6301_RS23345 (K6301_23305) 1535894..1536325 - 432 WP_247221213.1 MarR family transcriptional regulator -
K6301_RS23350 (K6301_23310) 1536488..1536802 - 315 WP_247221214.1 putative addiction module antidote protein Antitoxin
K6301_RS23355 (K6301_23315) 1536807..1537103 - 297 WP_247221215.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6301_RS23360 (K6301_23320) 1537242..1537754 - 513 WP_247221216.1 hypothetical protein -
K6301_RS23365 (K6301_23325) 1538164..1538385 + 222 WP_247222500.1 helix-turn-helix domain-containing protein -
K6301_RS23370 (K6301_23330) 1538827..1539006 - 180 WP_247221217.1 hypothetical protein -
K6301_RS23375 (K6301_23335) 1539965..1540195 + 231 WP_247221218.1 hypothetical protein -
K6301_RS23380 (K6301_23340) 1540652..1541266 + 615 Protein_1432 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - adeG 1..1888955 1888955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-91)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-89)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11118.83 Da        Isoelectric Point: 10.4464

>T213833 WP_247221215.1 NZ_CP081988:c1537103-1536807 [Shinella oryzae]
MFTIRRTRIFADWIKALKDFRAQARIADRIDRLAAGNSGDAKPIGEGVSELRINYGPGYRVYFVRDGSIVYLLLCGGDKS
TQDKDIRLAKKLAQQLRE

Download         Length: 297 bp

>T213833 NZ_CP109875:25608-25742 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 10788.25 Da        Isoelectric Point: 7.2005

>AT213833 WP_247221214.1 NZ_CP081988:c1536802-1536488 [Shinella oryzae]
MPLETFEYDSAEFLGSEEAIAEYLLAASADGDANHIARALGVVARARGMSDLSRKTGLTRPALYRALSGHGNPELGTIAK
VAGALGYRLNLVLKSASGSAPAVK

Download         Length: 315 bp

>AT213833 NZ_CP109875:c25563-25503 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References