Detailed information of TA system TA213830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1401204..1401802
• Replicon: plasmid pZ25
• Accession: NZ_CP081988
• Organism: Shinella oryzae strain Z-25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6301_RS22710
• Protein ID: WP_247221107.1
• Coordinates: 1401509..1401802 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K6301_RS22705
• Protein ID: WP_044460133.1
• Coordinates: 1401204..1401512 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6301_RS22680 (K6301_22660)	1396543..1397193	-	651	WP_247221102.1	hypothetical protein	-
K6301_RS22685 (K6301_22665)	1397537..1397926	-	390	WP_247221103.1	hypothetical protein	-
K6301_RS22690 (K6301_22670)	1397923..1399065	-	1143	WP_247221104.1	C2H2-type zinc finger protein	-
K6301_RS22695	1399110..1399544	-	435	WP_247221105.1	endonuclease domain-containing protein	-
K6301_RS22700	1399604..1400443	-	840	WP_247221106.1	hypothetical protein	-
K6301_RS22705 (K6301_22680)	1401204..1401512	+	309	WP_044460133.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K6301_RS22710 (K6301_22685)	1401509..1401802	+	294	WP_247221107.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6301_RS22715 (K6301_22690)	1402009..1403292	+	1284	WP_281505888.1	extracellular solute-binding protein	-
K6301_RS22720 (K6301_22695)	1403304..1405205	+	1902	WP_247221109.1	ATP-binding protein	-
K6301_RS22725 (K6301_22700)	1405202..1406527	+	1326	WP_247221110.1	sigma-54 dependent transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..1888955	1888955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10510.00 Da
• Isoelectric Point: 5.7996

>T213830 WP_247221107.1 NZ_CP081988:1401509-1401802 [Shinella oryzae]
VKLTWSAFALSDRDAIFTYIEADNPAAAVLIDERIAAGARRLLDFPASGRVGRIAGTRELVISGTPYIAAYTAAETTVRI
LRVLHGAQEWPENLPLS

Nucleotide

• Length: 294 bp

>T213830 NZ_CP109874:c4736416-4736030 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11046.42 Da
• Isoelectric Point: 4.8892

>AT213830 WP_044460133.1 NZ_CP081988:1401204-1401512 [Shinella oryzae]
MAANALVQTRIDSEVRDRASAVLESMGLTVSDAVRIMLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDLSDD
EAEAHFAKRRAAARLKAGVPES

Nucleotide

• Length: 309 bp

>AT213830 NZ_CP109874:c4737018-4736446 [Escherichia coli]
ATGTTTTGCGAAGAAAAAGTAGCTCAAATGGCTGCATACCTACTTCTTAAGCGAGGTGGGCGCATGGCATATCTGAAATT
GATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGAAGATAGTCTTTACTCTA
TGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGCATTGGTGACTACTGGTACAAC
TTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGACGAGGTCTTTGATGAGTT
GAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATTTGATCTCGCAAACATGA
CGCATTTAGAAAGCGTTTGTCCAGAGTGGCATAATCCTGGCAATTCTCGTAAGCCTATAGACCTGAAAGAAATGCTGATC
AGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAACTTAAGGAATTTTCTTT
GCAATTATCATGA