Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 181360..181946 Replicon chromosome
Accession NZ_CP081987
Organism Shinella oryzae strain Z-25

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag K6301_RS00910 Protein ID WP_247218690.1
Coordinates 181360..181689 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag K6301_RS00915 Protein ID WP_247218692.1
Coordinates 181689..181946 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6301_RS00885 (K6301_00880) 176477..176716 - 240 WP_247218682.1 30S ribosomal protein S21 -
K6301_RS00890 (K6301_00885) 176781..177071 - 291 WP_247218684.1 cold-shock protein -
K6301_RS00895 (K6301_00890) 177201..177410 - 210 WP_134650825.1 cold-shock protein -
K6301_RS00900 (K6301_00895) 177747..179957 - 2211 WP_247218686.1 FAD-dependent oxidoreductase -
K6301_RS00905 (K6301_00900) 180110..181318 - 1209 WP_247218688.1 acetyl-CoA C-acetyltransferase -
K6301_RS00910 (K6301_00905) 181360..181689 - 330 WP_247218690.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K6301_RS00915 (K6301_00910) 181689..181946 - 258 WP_247218692.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
K6301_RS00920 (K6301_00915) 182006..183802 - 1797 WP_247218695.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 161021..181946 20925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

SpoVT-AbrB domain

(9-51)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11864.68 Da        Isoelectric Point: 10.0069

>T213827 WP_247218690.1 NZ_CP081987:c181689-181360 [Shinella oryzae]
MDRGDIWFADLEPTRGREQAKARYVLILSPRPFNNLGVQIIAPITSGGEFARAKGFTVSLSGAGTQANGVILCHQMRSID
IKARGGRFVEKAPAFIVEEALARVAAFFE

Download         Length: 330 bp

>T213827 NZ_CP109874:c4467349-4466999 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.84 Da        Isoelectric Point: 4.2346

>AT213827 WP_247218692.1 NZ_CP081987:c181946-181689 [Shinella oryzae]
MATSTLRNVGGSVMMTVPKPVLEELGIEANTKLDISVQDGKIVAIPRKRPKYTLEELIAQCDFDAPWSEEEREWMDAPAV
GNEII

Download         Length: 258 bp

>AT213827 NZ_CP109874:c4467594-4467343 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References