Detailed information of TA system TA213826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 8223..8821
• Replicon: plasmid unnamed
• Accession: NZ_CP081967
• Organism: Pseudomonas mosselii strain DSM 17497

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6H1Q9F7
• Locus tag: K5H97_RS29170
• Protein ID: WP_028692294.1
• Coordinates: 8519..8821 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K5H97_RS29165
• Protein ID: WP_028692295.1
• Coordinates: 8223..8522 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5H97_RS29145 (K5H97_29145)	3594..4877	-	1284	WP_028691924.1	Y-family DNA polymerase	-
K5H97_RS29150 (K5H97_29150)	4867..5295	-	429	WP_028691923.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
K5H97_RS29155 (K5H97_29155)	7364..7687	-	324	WP_028692297.1	hypothetical protein	-
K5H97_RS29160 (K5H97_29160)	7982..8203	-	222	WP_028692296.1	hypothetical protein	-
K5H97_RS29165 (K5H97_29165)	8223..8522	-	300	WP_028692295.1	putative addiction module antidote protein	Antitoxin
K5H97_RS29170 (K5H97_29170)	8519..8821	-	303	WP_028692294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5H97_RS29175 (K5H97_29175)	9024..9803	+	780	WP_028692293.1	DUF4942 domain-containing protein	-
K5H97_RS29180 (K5H97_29180)	9956..10324	+	369	WP_028692292.1	hypothetical protein	-
K5H97_RS29185 (K5H97_29185)	10351..10668	+	318	WP_028692291.1	hypothetical protein	-
K5H97_RS29190 (K5H97_29190)	11006..12097	+	1092	WP_051555741.1	hypothetical protein	-
K5H97_RS29195 (K5H97_29195)	12243..12485	+	243	WP_028692289.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..65474	65474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11277.85 Da
• Isoelectric Point: 10.3903

>T213826 WP_028692294.1 NZ_CP081967:c8821-8519 [Pseudomonas mosselii]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKSLASQWSDDE

Nucleotide

• Length: 303 bp

>T213826 NZ_CP109874:4349596-4349853 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10825.27 Da
• Isoelectric Point: 5.1084

>AT213826 WP_028692295.1 NZ_CP081967:c8522-8223 [Pseudomonas mosselii]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPAH

Nucleotide

• Length: 300 bp

>AT213826 NZ_CP109874:4349865-4350410 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: A0A6H1Q9F7