Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 729074..729714 Replicon chromosome
Accession NZ_CP081966
Organism Pseudomonas mosselii strain DSM 17497

Toxin (Protein)


Gene name vapC Uniprot ID A0A1H9S264
Locus tag K5H97_RS03285 Protein ID WP_028691288.1
Coordinates 729074..729484 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A246GUY5
Locus tag K5H97_RS03290 Protein ID WP_036986184.1
Coordinates 729484..729714 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5H97_RS03260 (K5H97_03260) 726072..726497 - 426 WP_028691293.1 DUF2946 domain-containing protein -
K5H97_RS03265 (K5H97_03265) 726523..727005 - 483 WP_028691292.1 copper chaperone PCu(A)C -
K5H97_RS03270 (K5H97_03270) 727053..727442 - 390 WP_028691291.1 DUF2946 domain-containing protein -
K5H97_RS03275 (K5H97_03275) 727683..728537 - 855 WP_028691290.1 SPFH domain-containing protein -
K5H97_RS03280 (K5H97_03280) 728564..729001 - 438 WP_028691289.1 NfeD family protein -
K5H97_RS03285 (K5H97_03285) 729074..729484 - 411 WP_028691288.1 tRNA(fMet)-specific endonuclease VapC Toxin
K5H97_RS03290 (K5H97_03290) 729484..729714 - 231 WP_036986184.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5H97_RS03295 (K5H97_03295) 729797..730525 - 729 WP_028691287.1 cobalt-precorrin-6A reductase -
K5H97_RS03300 (K5H97_03300) 730522..731616 - 1095 WP_028691286.1 cobalt-precorrin-5B (C(1))-methyltransferase -
K5H97_RS03305 (K5H97_03305) 731609..732820 - 1212 WP_028691285.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
K5H97_RS03310 (K5H97_03310) 732988..734313 + 1326 WP_028691284.1 precorrin-3B synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15234.59 Da        Isoelectric Point: 9.5447

>T213824 WP_028691288.1 NZ_CP081966:c729484-729074 [Pseudomonas mosselii]
MLRYMLDTNICIFTIKNKPQVVREAFNRHHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTHPR

Download         Length: 411 bp

>T213824 NZ_CP109874:c3936853-3936455 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8459.36 Da        Isoelectric Point: 4.2821

>AT213824 WP_036986184.1 NZ_CP081966:c729714-729484 [Pseudomonas mosselii]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSSDFMASREQPADQEREGF

Download         Length: 231 bp

>AT213824 NZ_CP109874:c3937149-3936856 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H9S264

Antitoxin

Source ID Structure
AlphaFold DB A0A246GUY5

References