Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09812-MazE
Location 2414019..2414650 Replicon chromosome
Accession NZ_CP081946
Organism Pseudomonas sp. DR48

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag K5K95_RS11045 Protein ID WP_222589465.1
Coordinates 2414288..2414650 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag K5K95_RS11040 Protein ID WP_222589464.1
Coordinates 2414019..2414288 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5K95_RS11005 (K5K95_11005) 2409558..2410103 - 546 WP_090176512.1 hypothetical protein -
K5K95_RS11010 (K5K95_11010) 2410100..2410354 - 255 WP_222589460.1 acyl carrier protein -
K5K95_RS11015 (K5K95_11015) 2410364..2410624 - 261 WP_090176507.1 phosphopantetheine-binding protein -
K5K95_RS11020 (K5K95_11020) 2410605..2411414 - 810 WP_222589461.1 lysophospholipid acyltransferase family protein -
K5K95_RS11025 (K5K95_11025) 2411390..2412115 - 726 WP_222589462.1 beta-ketoacyl synthase chain length factor -
K5K95_RS11030 (K5K95_11030) 2412471..2413241 + 771 WP_222589463.1 ParA family protein -
K5K95_RS11035 (K5K95_11035) 2413312..2413746 - 435 WP_046045645.1 thioredoxin TrxC -
K5K95_RS11040 (K5K95_11040) 2414019..2414288 + 270 WP_222589464.1 PbsX family transcriptional regulator Antitoxin
K5K95_RS11045 (K5K95_11045) 2414288..2414650 + 363 WP_222589465.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K5K95_RS11050 (K5K95_11050) 2414657..2416120 - 1464 WP_222589466.1 YdiU family protein -
K5K95_RS11055 (K5K95_11055) 2416169..2419516 - 3348 WP_222589467.1 mechanosensitive channel MscK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13226.07 Da        Isoelectric Point: 6.4635

>T213822 WP_222589465.1 NZ_CP081946:2414288-2414650 [Pseudomonas sp. DR48]
MVRRQAPQRGDVYWIDPNPVAGREMMDRHRFVVITPREINSLGVSMTVPVTSGGSFSRSSGLAVIVSGHETNGVAVCNQV
RSFDIEQRVRDGSAKFIERLDDITMADIIARVVSAIDPLE

Download         Length: 363 bp

>T213822 NZ_CP109874:c3712596-3712294 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9606.02 Da        Isoelectric Point: 5.0931

>AT213822 WP_222589464.1 NZ_CP081946:2414019-2414288 [Pseudomonas sp. DR48]
MSVTSRIRRQGGAAVITLPPTLLKLLHLDVGAQLELNVVDGELIARPVIQSSRKRYSLAELLEGSEEIAELNAQTAFARE
GDPVGRELG

Download         Length: 270 bp

>AT213822 NZ_CP109874:c3712258-3711917 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References