Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1860333..1861960 Replicon chromosome
Accession NZ_CP081946
Organism Pseudomonas sp. DR48

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag K5K95_RS08560 Protein ID WP_222589091.1
Coordinates 1860608..1861960 (-) Length 451 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag K5K95_RS08555 Protein ID WP_222589090.1
Coordinates 1860333..1860611 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5K95_RS08525 (K5K95_08525) 1855701..1856324 + 624 WP_222589084.1 FMN-binding negative transcriptional regulator -
K5K95_RS08530 (K5K95_08530) 1856335..1856781 + 447 WP_222589085.1 GNAT family N-acetyltransferase -
K5K95_RS08535 (K5K95_08535) 1856799..1857470 + 672 WP_222589086.1 GNAT family protein -
K5K95_RS08540 (K5K95_08540) 1857539..1858438 - 900 WP_222589087.1 homocysteine S-methyltransferase family protein -
K5K95_RS08545 (K5K95_08545) 1858551..1859372 + 822 WP_222589088.1 ABC transporter substrate-binding protein -
K5K95_RS08550 (K5K95_08550) 1859369..1860205 + 837 WP_222589089.1 amino acid ABC transporter permease -
K5K95_RS08555 (K5K95_08555) 1860333..1860611 - 279 WP_222589090.1 helix-turn-helix transcriptional regulator Antitoxin
K5K95_RS08560 (K5K95_08560) 1860608..1861960 - 1353 WP_222589091.1 HipA domain-containing protein Toxin
K5K95_RS08565 (K5K95_08565) 1862348..1864555 + 2208 WP_222589092.1 autotransporter outer membrane beta-barrel domain-containing protein -
K5K95_RS08570 (K5K95_08570) 1864631..1866454 - 1824 WP_222589093.1 sodium-extruding oxaloacetate decarboxylase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - algR / algC / vipB/mglB 1624297..1870616 246319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(165-367)

Antitoxin

Cro/C1-type helix-turn-helix domain

(27-80)


Sequences

Toxin        


Download         Length: 451 a.a.        Molecular weight: 50072.69 Da        Isoelectric Point: 5.1423

>T213821 WP_222589091.1 NZ_CP081946:c1861960-1860608 [Pseudomonas sp. DR48]
MYTLTLQIFAAGIWRDAMDLNFDSPEKGFESRCNFGYETPYLVESIEDIGSPFAKAVSARFPLDFEGKRSNAPAFLHDIA
PAGAAKRFLLAHLGRDKPADVSADLYLLARSTPAPIGNMRIKESAEAVDQREPIGFKRQDVISRDNRFLEYAYEQGAAIG
GATGAGGEAPKLLLAQGKDGNLYPDAVLDDAAVTQHWFVKFSRGKGTRNDQDILRSEYHYYKALQKLGIETVAEQGLALE
EATKPSLWMHRFDRQVTPQGVERFAVESIYSLAQITTPGSPMDHMEVIRLLAGLWRDAGQADQIPNLVADYLRRDLLNKI
LGNSDNHGRNTAIIRDTASFRLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVNWRGVCQALADIADPNDPLAALIDLP
GSFERLREDARHLLALPDILTDSGLPDATMNHPRIALKNLEQRLKEWDLK

Download         Length: 1353 bp

>T213821 NZ_CP109874:3297030-3297416 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10290.04 Da        Isoelectric Point: 10.7364

>AT213821 WP_222589090.1 NZ_CP081946:c1860611-1860333 [Pseudomonas sp. DR48]
MSMTVAERTVLIESIQEALAQGTLEIGEAVRRLRVEVTGLHQTQFAKMCKVSVRTLVHIEHGEGNQTLKSLNSVFRPFGL
KMGVVRVRRDLS

Download         Length: 279 bp

>AT213821 NZ_CP109874:3297406-3297735 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References