Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-DUF971
Location 5350..5945 Replicon plasmid pDR208
Accession NZ_CP081945
Organism Pseudomonas sp. DR208

Toxin (Protein)


Gene name PumA Uniprot ID G9G7G3
Locus tag K5K89_RS28350 Protein ID WP_011475355.1
Coordinates 5643..5945 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID G9G7G2
Locus tag K5K89_RS28345 Protein ID WP_011475354.1
Coordinates 5350..5646 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5K89_RS28325 (K5K89_28325) 638..1147 + 510 WP_222541776.1 hypothetical protein -
K5K89_RS28330 (K5K89_28330) 1158..2426 + 1269 WP_222541777.1 DUF1173 family protein -
K5K89_RS28335 (K5K89_28335) 3058..4341 - 1284 WP_222541778.1 Y-family DNA polymerase -
K5K89_RS28340 (K5K89_28340) 4331..4759 - 429 WP_222541779.1 S24 family peptidase -
K5K89_RS28345 (K5K89_28345) 5350..5646 - 297 WP_011475354.1 putative addiction module antidote protein Antitoxin
K5K89_RS28350 (K5K89_28350) 5643..5945 - 303 WP_011475355.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K5K89_RS28355 (K5K89_28355) 6155..6373 + 219 WP_219022106.1 hypothetical protein -
K5K89_RS28360 (K5K89_28360) 6437..6598 + 162 WP_169882213.1 hypothetical protein -
K5K89_RS28365 (K5K89_28365) 6793..7089 + 297 WP_222541780.1 hypothetical protein -
K5K89_RS28370 (K5K89_28370) 7095..7388 + 294 WP_222541781.1 hypothetical protein -
K5K89_RS28375 (K5K89_28375) 7510..7752 + 243 WP_110973482.1 hypothetical protein -
K5K89_RS28380 (K5K89_28380) 7804..9486 + 1683 WP_222541782.1 DNA cytosine methyltransferase -
K5K89_RS28385 (K5K89_28385) 9568..9786 + 219 WP_110973480.1 hypothetical protein -
K5K89_RS28390 (K5K89_28390) 9798..10217 + 420 WP_222541783.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..41441 41441


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(9-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11247.96 Da        Isoelectric Point: 9.9233

>T213819 WP_011475355.1 NZ_CP081945:c5945-5643 [Pseudomonas sp. DR208]
MKTIIQTATYMTWERKLRDKKAKLIIAARVLRVAHGLLGDVQPVGQGVSELRIHHGPGYRVYFQQRGDQLVLLLCGGDKS
SQARDIETAKTLASQWSEDE

Download         Length: 303 bp

>T213819 NZ_CP109874:2879982-2880089 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10482.08 Da        Isoelectric Point: 5.5740

>AT213819 WP_011475354.1 NZ_CP081945:c5646-5350 [Pseudomonas sp. DR208]
MKEPIYEYDPAELLTDAESIAIFMADAFESGDASFIAQALSVVARAKGMTAIAKEAGVTRAHLYQLKNGNPTLKTMIGVM
RAVGLDMTAKQHVDVAHG

Download         Length: 297 bp

>AT213819 NZ_CP109874:c2879932-2879875 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G9G7G3

Antitoxin

Source ID Structure
AlphaFold DB G9G7G2

References