Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2208630..2209234 Replicon chromosome
Accession NZ_CP081944
Organism Pseudomonas sp. DR208

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag K5K89_RS09965 Protein ID WP_222541321.1
Coordinates 2208630..2208818 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1H0W5P5
Locus tag K5K89_RS09970 Protein ID WP_071488481.1
Coordinates 2208830..2209234 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5K89_RS09935 (K5K89_09935) 2203986..2204522 + 537 WP_071488488.1 DsbE family thiol:disulfide interchange protein -
K5K89_RS09940 (K5K89_09940) 2204519..2204992 + 474 WP_071488487.1 cytochrome c-type biogenesis protein CcmH -
K5K89_RS09945 (K5K89_09945) 2204985..2206169 + 1185 WP_222541318.1 c-type cytochrome biogenesis protein CcmI -
K5K89_RS09950 (K5K89_09950) 2206185..2206577 + 393 WP_071488485.1 hypothetical protein -
K5K89_RS09955 (K5K89_09955) 2206959..2207531 + 573 WP_222541319.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
K5K89_RS09960 (K5K89_09960) 2207561..2208439 - 879 WP_222541320.1 LysR family transcriptional regulator -
K5K89_RS09965 (K5K89_09965) 2208630..2208818 + 189 WP_222541321.1 type II toxin-antitoxin system HicA family toxin Toxin
K5K89_RS09970 (K5K89_09970) 2208830..2209234 + 405 WP_071488481.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K5K89_RS09975 (K5K89_09975) 2209609..2211138 + 1530 WP_261401095.1 M57 family metalloprotease -
K5K89_RS09980 (K5K89_09980) 2211370..2213079 + 1710 WP_222541322.1 M10 family metallopeptidase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7207.45 Da        Isoelectric Point: 10.8676

>T213816 WP_222541321.1 NZ_CP081944:2208630-2208818 [Pseudomonas sp. DR208]
VQSRLLIKELQDAGWVLDRVTGSHHIFIHRYSLYTIPVPHPKKDLPLGTLRSIRKRAGLFDF

Download         Length: 189 bp

>T213816 NZ_CP109874:2196463-2196566 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14773.78 Da        Isoelectric Point: 4.6017

>AT213816 WP_071488481.1 NZ_CP081944:2208830-2209234 [Pseudomonas sp. DR208]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGTIPMPTSVARYHAHEDYAGMGWGM
LELDVTPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT213816 NZ_CP109874:c2196604-2196333 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1H0W5P5

References