Detailed information of TA system TA213815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1821594..1822209
• Replicon: chromosome
• Accession: NZ_CP081944
• Organism: Pseudomonas sp. DR208

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K5K89_RS08030
• Protein ID: WP_222541167.1
• Coordinates: 1822027..1822209 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1H0VRD6
• Locus tag: K5K89_RS08025
• Protein ID: WP_071488915.1
• Coordinates: 1821594..1822004 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5K89_RS08000 (K5K89_08000)	1817416..1818357	+	942	WP_222541165.1	hydroxymethylglutaryl-CoA lyase	-
K5K89_RS08005 (K5K89_08005)	1818417..1819748	+	1332	WP_222541166.1	MFS transporter	-
K5K89_RS08010 (K5K89_08010)	1819913..1820440	+	528	WP_071488912.1	DUF4142 domain-containing protein	-
K5K89_RS08015 (K5K89_08015)	1820496..1820921	-	426	WP_092241350.1	low affinity iron permease family protein	-
K5K89_RS08020 (K5K89_08020)	1821003..1821503	-	501	WP_092241352.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
K5K89_RS08025 (K5K89_08025)	1821594..1822004	-	411	WP_071488915.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K5K89_RS08030 (K5K89_08030)	1822027..1822209	-	183	WP_222541167.1	type II toxin-antitoxin system HicA family toxin	Toxin
K5K89_RS08035 (K5K89_08035)	1822378..1823271	-	894	WP_222541168.1	LysR family transcriptional regulator	-
K5K89_RS08040 (K5K89_08040)	1823367..1824530	+	1164	WP_222541169.1	MFS transporter	-
K5K89_RS08045 (K5K89_08045)	1824527..1825696	+	1170	WP_222541170.1	MFS transporter	-
K5K89_RS28610	1825685..1825807	-	123	Protein_1590	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6857.08 Da
• Isoelectric Point: 10.9586

>T213815 WP_222541167.1 NZ_CP081944:c1822209-1822027 [Pseudomonas sp. DR208]
VDSRYLIGRIVADGWYLVRIRGSHHHFKHPTKPGLVTIPHPKKDLFDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T213815 NZ_CP109874:1922456-1922710 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAATGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.63 Da
• Isoelectric Point: 4.3883

>AT213815 WP_071488915.1 NZ_CP081944:c1822004-1821594 [Pseudomonas sp. DR208]
MLYPIAISTGDDDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQDH

Nucleotide

• Length: 411 bp

>AT213815 NZ_CP109874:1922208-1922459 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1H0VRD6