Detailed information of TA system TA213813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 6459822..6460861
• Replicon: chromosome
• Accession: NZ_CP081943
• Organism: Pseudomonas sp. MM213

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: K5R88_RS29425
• Protein ID: WP_226298829.1
• Coordinates: 6459822..6460397 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: K5R88_RS29430
• Protein ID: WP_223449629.1
• Coordinates: 6460394..6460861 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5R88_RS29400 (K5R88_29280)	6455160..6457148	+	1989	WP_207285363.1	heme lyase CcmF/NrfE family subunit	-
K5R88_RS29405 (K5R88_29285)	6457145..6457681	+	537	WP_226298828.1	DsbE family thiol:disulfide interchange protein	-
K5R88_RS29410 (K5R88_29290)	6457678..6458151	+	474	WP_192226062.1	cytochrome c-type biogenesis protein CcmH	-
K5R88_RS29415 (K5R88_29295)	6458144..6459346	+	1203	WP_008039841.1	c-type cytochrome biogenesis protein CcmI	-
K5R88_RS29420 (K5R88_29300)	6459361..6459765	+	405	WP_008039842.1	hypothetical protein	-
K5R88_RS29425 (K5R88_29305)	6459822..6460397	-	576	WP_226298829.1	PIN domain-containing protein	Toxin
K5R88_RS29430 (K5R88_29310)	6460394..6460861	-	468	WP_223449629.1	helix-turn-helix domain-containing protein	Antitoxin
K5R88_RS29435 (K5R88_29315)	6460943..6461212	-	270	Protein_5802	LysR substrate-binding domain-containing protein	-
K5R88_RS29440 (K5R88_29320)	6461252..6461971	-	720	WP_008026798.1	SDR family oxidoreductase	-
K5R88_RS29445 (K5R88_29325)	6462253..6462465	+	213	WP_081501757.1	type II toxin-antitoxin system HicA family toxin	-
K5R88_RS29450 (K5R88_29330)	6462465..6462869	+	405	WP_192226071.1	type II toxin-antitoxin system HicB family antitoxin	-
K5R88_RS29455 (K5R88_29335)	6462968..6464173	-	1206	WP_226298830.1	MFS transporter	-
K5R88_RS29460 (K5R88_29340)	6464388..6465005	+	618	WP_226298831.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21669.83 Da
• Isoelectric Point: 5.0417

>T213813 WP_226298829.1 NZ_CP081943:c6460397-6459822 [Pseudomonas sp. MM213]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGRFRAKWTMEIHNEWKRNLLKNRPDLTTGQLNRTSELMDQALPDACVSGY
EELIEGLTLPDVDDRHVLAVAIHCNASVIVTFNQKDFPCTALSPFGIEAQHPDEFVDNLFDLDPAAVIEAAQRQRKQLKL
PPMDVDSYLELLLRQGLVQSSKALTEYRAIL

Nucleotide

• Length: 576 bp

>T213813 NZ_CP109874:1085405-1085740 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17339.76 Da
• Isoelectric Point: 6.2264

>AT213813 WP_223449629.1 NZ_CP081943:c6460861-6460394 [Pseudomonas sp. MM213]
MSPTLHPTINLPLEREVQAAIEGQRALAAYLATQFETQRIQIFDDRNEAHSVELPTSALRLLVDILEALAAGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESGDLSYHKTGKHRRVRFADLMDYKNRRDTASEQAMMLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT213813 NZ_CP109874:1085157-1085405 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA