Detailed information of TA system TA213810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2658354..2659772
• Replicon: chromosome
• Accession: NZ_CP081942
• Organism: Pseudomonas sp. MM211

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K5Q02_RS12140
• Protein ID: WP_225830807.1
• Coordinates: 2658354..2658923 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K5Q02_RS12145
• Protein ID: WP_225830809.1
• Coordinates: 2658930..2659772 (+)
• Length: 281 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Q02_RS12130 (K5Q02_12085)	2654147..2654488	-	342	WP_225830805.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K5Q02_RS12135 (K5Q02_12090)	2654709..2658184	-	3476	Protein_2395	hybrid sensor histidine kinase/response regulator	-
K5Q02_RS12140 (K5Q02_12095)	2658354..2658923	+	570	WP_225830807.1	GNAT family protein	Toxin
K5Q02_RS12145 (K5Q02_12100)	2658930..2659772	+	843	WP_225830809.1	AraC family transcriptional regulator	Antitoxin
K5Q02_RS12150 (K5Q02_12105)	2659951..2660661	+	711	WP_225830811.1	AzlC family ABC transporter permease	-
K5Q02_RS12155 (K5Q02_12110)	2660661..2660990	+	330	WP_225830813.1	AzlD domain-containing protein	-
K5Q02_RS12160 (K5Q02_12115)	2661121..2661738	+	618	WP_225830815.1	glutathione S-transferase	-
K5Q02_RS12165 (K5Q02_12120)	2661777..2663843	+	2067	WP_225830817.1	pyridoxamine 5'-phosphate oxidase family protein	-
K5Q02_RS12170 (K5Q02_12125)	2663913..2664377	+	465	WP_225830819.1	DUF1348 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (193-270)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21635.00 Da
• Isoelectric Point: 10.1215

>T213810 WP_225830807.1 NZ_CP081942:2658354-2658923 [Pseudomonas sp. MM211]
MFKPQLITLQRGALRIEPLLDGDIPALVTLAEVNRSELAYMSGTRRLDWYRQALHEQREDRALPFVIRLGDQLVGTTRFA
DFIPTLPAVEIGWTWLDASQHGTGLNSMIKYLMLRHAFESWRMVRVQLKTAGSNLRSQRAIEKLGAIREGLLRNHRRLAD
GRLDDTVMYSITDHEWPVVKAGLEARFGV

Nucleotide

• Length: 570 bp

>T213810 NZ_CP109874:c618831-618367 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 281 a.a.
• Molecular weight: 31075.33 Da
• Isoelectric Point: 8.7307

>AT213810 WP_225830809.1 NZ_CP081942:2658930-2659772 [Pseudomonas sp. MM211]
MDSGERTRFWESSALAGVELLQARYIKQRFTPHVHEGFVFTVIEHGAQRFRHRGADHLAPIGSMVLINPDEVHTGSAAHE
QGWFYRAFYPAPAQVNGVLEELGLAQRGLPSFAASVLQDVQLHQAFGQLHRLLDSEASKLQQQTAWRETLLMLFQRHAGV
SQAPLPGQEPRAVTLAKELLASRLGEPPSLEELAAAVNLSPFHFARVFRRSTGLPPHAWLQLRRLEQARALLRDGCAPLN
VALQLGFSDQSHLGRQFKQAYGVAPGEYRNACQRSPVACG

Nucleotide

• Length: 843 bp

>AT213810 NZ_CP109874:c619166-618831 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA