Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5168390..5169015 Replicon chromosome
Accession NZ_CP081897
Organism Klebsiella pneumoniae strain MN-03

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KZ659_RS25235 Protein ID WP_002882817.1
Coordinates 5168390..5168773 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KZ659_RS25240 Protein ID WP_004150355.1
Coordinates 5168773..5169015 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ659_RS25220 (KZ659_25145) 5165756..5166658 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KZ659_RS25225 (KZ659_25150) 5166655..5167290 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KZ659_RS25230 (KZ659_25155) 5167287..5168216 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KZ659_RS25235 (KZ659_25160) 5168390..5168773 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ659_RS25240 (KZ659_25165) 5168773..5169015 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KZ659_RS25245 (KZ659_25170) 5169220..5170137 + 918 WP_004895173.1 alpha/beta hydrolase -
KZ659_RS25250 (KZ659_25175) 5170151..5171092 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KZ659_RS25255 (KZ659_25180) 5171137..5171574 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KZ659_RS25260 (KZ659_25185) 5171571..5172431 - 861 WP_004146232.1 virulence factor BrkB family protein -
KZ659_RS25265 (KZ659_25190) 5172425..5173024 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213796 WP_002882817.1 NZ_CP081897:c5168773-5168390 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213796 NZ_CP109854:603063-603395 [Pantoea dispersa]
ATGGTGTCACGTTTTGTTCCCGATGCAGGCGATCTCATCTGGCTGGATTTTGATCCCACCCTTGGCCATGAACAGGGCGG
CCATCGTCCTGCGGTTGTGCTGAGTCCCTTTGCCTATAACAACAAGGTCGGCATGTTGTTGTGCGTCCCGTGTACCACCC
AGGTCAAAGGCTATCCTTTCGAAGTTTCCCTTGTCGGTAGCCGTGAAAGTGTGGCGCTGGCCGATCAGGTCACCTCCATC
GACTGGCGCGCCAGAAAAGTGGTGAAAAAAAGCCATGTGACCGCCGACGAACTGAGTGAGATTCGCGCTAAAGCTAAAGC
GCTGATTGGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213796 WP_004150355.1 NZ_CP081897:c5169015-5168773 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213796 NZ_CP109854:602818-603063 [Pantoea dispersa]
ATGACGACCGTCACCTTAAAGAAATGGGGCAACAGTCCCTCGATTCGCATCCCATCCAGCGTGATGCAATCCGCGTCGCT
CAGCGTGAACGATAAACTGGAACTCAGCGTTGAAGATGGCAAGCTGATCCTTGTGCCGCTCAGGGAAAAGGTGTATTCAC
TGGACGCTCTGCTGGACGAGATCACCGACGATAACGTCCATGATAAAATTGATTTTGGACCTTCAGTGGGCAAGGAAAGA
ATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References