Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4572248..4573058 Replicon chromosome
Accession NZ_CP081897
Organism Klebsiella pneumoniae strain MN-03

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KZ659_RS22415 Protein ID WP_004178461.1
Coordinates 4572248..4572781 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KZ659_RS22420 Protein ID WP_002887278.1
Coordinates 4572792..4573058 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ659_RS22410 (KZ659_22330) 4571079..4572200 + 1122 WP_002887282.1 cupin domain-containing protein -
KZ659_RS22415 (KZ659_22335) 4572248..4572781 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KZ659_RS22420 (KZ659_22340) 4572792..4573058 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KZ659_RS22425 (KZ659_22345) 4573161..4574594 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
KZ659_RS22430 (KZ659_22350) 4574584..4575267 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KZ659_RS22435 (KZ659_22355) 4575439..4576824 + 1386 WP_004894567.1 efflux transporter outer membrane subunit -
KZ659_RS22440 (KZ659_22360) 4576842..4577186 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T213793 WP_004178461.1 NZ_CP081897:c4572781-4572248 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213793 NZ_CP109854:c309261-308974 [Pantoea dispersa]
ATGGCCTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAATTAAA
AAAAAAGCTGGCTGAAGTACTTGAGGCACCCCGTATTGAAGCGAACAAGCTTCGCGGTATGCCTGACTGCTACAAAATAA
AGCTCAGGTCTTCAGGTTATCGCCTTGTCTATCAGGTGATAGACGAGAAGGTGGTGGTTTTCGTGATTTCGGTTGGTAAA
AGAGAACGCGCGGAGGTTTATAACGAGGCCGTGAAGCGCATTCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213793 WP_002887278.1 NZ_CP081897:c4573058-4572792 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213793 NZ_CP109854:c309500-309261 [Pantoea dispersa]
ATGGGCAGCATCAACCTGCGTATTGATGATGAACTCAAAGCACGTTCTTACGCTGCGCTTGAAAAAATGGGGGTGACTCC
CTCTGAAGCGCTTCGTCTCATGCTTGAGTATATCGCCGAAAATGAACGTTTACCGTTCAAACAGACACTGCTGAGCGACG
AAGATGCTGAGCTGGTCGAAATAGTGAAAGAGCGGCTTCGCAATCCTAAGCCAGTTCGTGTGACGCTGGATGACCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References