Detailed information of TA system TA213774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4541431..4542241
• Replicon: chromosome
• Accession: NZ_CP081896
• Organism: Klebsiella pneumoniae strain PA-01

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A060VJ83
• Locus tag: KZ665_RS22295
• Protein ID: WP_004178461.1
• Coordinates: 4541431..4541964 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: KZ665_RS22300
• Protein ID: WP_102008156.1
• Coordinates: 4541975..4542241 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ665_RS22290 (KZ665_22215)	4540262..4541383	+	1122	WP_002887282.1	cupin domain-containing protein	-
KZ665_RS22295 (KZ665_22220)	4541431..4541964	-	534	WP_004178461.1	type II toxin-antitoxin system toxin KacT	Toxin
KZ665_RS22300 (KZ665_22225)	4541975..4542241	-	267	WP_102008156.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
KZ665_RS22305 (KZ665_22230)	4542344..4543777	-	1434	WP_102008157.1	Cu(+)/Ag(+) sensor histidine kinase	-
KZ665_RS22310 (KZ665_22235)	4543767..4544450	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
KZ665_RS22315 (KZ665_22240)	4544622..4546004	+	1383	WP_223051120.1	efflux transporter outer membrane subunit	-
KZ665_RS22320 (KZ665_22245)	4546022..4546366	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19810.65 Da
• Isoelectric Point: 5.2614

>T213774 WP_004178461.1 NZ_CP081896:c4541964-4541431 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T213774 NZ_CP109850:3468886-3469164 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGGCTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCAGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9964.40 Da
• Isoelectric Point: 10.1757

>AT213774 WP_102008156.1 NZ_CP081896:c4542241-4541975 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRVVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT213774 NZ_CP109850:3469176-3469481 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A060VJ83