Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5207953..5208578 Replicon chromosome
Accession NZ_CP081894
Organism Klebsiella pneumoniae strain NC-01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KZ666_RS25390 Protein ID WP_002882817.1
Coordinates 5207953..5208336 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KZ666_RS25395 Protein ID WP_004150355.1
Coordinates 5208336..5208578 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ666_RS25375 (5205319) 5205319..5206221 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KZ666_RS25380 (5206218) 5206218..5206853 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KZ666_RS25385 (5206850) 5206850..5207779 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KZ666_RS25390 (5207953) 5207953..5208336 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ666_RS25395 (5208336) 5208336..5208578 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KZ666_RS25400 (5208783) 5208783..5209700 + 918 WP_023286983.1 alpha/beta hydrolase -
KZ666_RS25405 (5209714) 5209714..5210655 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KZ666_RS25410 (5210700) 5210700..5211137 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KZ666_RS25415 (5211134) 5211134..5211994 - 861 WP_004146232.1 virulence factor BrkB family protein -
KZ666_RS25420 (5211988) 5211988..5212587 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213744 WP_002882817.1 NZ_CP081894:c5208336-5207953 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213744 NZ_CP109843:c5668432-5668154 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213744 WP_004150355.1 NZ_CP081894:c5208578-5208336 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213744 NZ_CP109843:c5668142-5667837 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References