Detailed information of TA system TA213725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2635041..2636629
• Replicon: chromosome
• Accession: NZ_CP081893
• Organism: Klebsiella quasipneumoniae strain WI-06

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KZ658_RS12845
• Protein ID: WP_044523012.1
• Coordinates: 2635041..2636363 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: KZ658_RS12850
• Protein ID: WP_023289667.1
• Coordinates: 2636363..2636629 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ658_RS12830 (2630091)	2630091..2632598	+	2508	WP_223065842.1	fimbria/pilus outer membrane usher protein	-
KZ658_RS12835 (2632586)	2632586..2633590	+	1005	WP_044523017.1	fimbrial protein	-
KZ658_RS12840 (2633732)	2633732..2635024	+	1293	WP_223065843.1	glycoside hydrolase family 10 protein	-
KZ658_RS12845 (2635041)	2635041..2636363	-	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
KZ658_RS12850 (2636363)	2636363..2636629	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KZ658_RS12855 (2636893)	2636893..2637219	-	327	WP_032452826.1	hydroxyisourate hydrolase	-
KZ658_RS12860 (2637216)	2637216..2637716	-	501	WP_223065844.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KZ658_RS12865 (2637694)	2637694..2639073	-	1380	WP_032452824.1	nucleobase:cation symporter-2 family protein	-
KZ658_RS12870 (2639419)	2639419..2640573	+	1155	WP_223065845.1	FAD-dependent urate hydroxylase HpxO	-
KZ658_RS12875 (2640554)	2640554..2641477	-	924	WP_023289662.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T213725 WP_044523012.1 NZ_CP081893:c2636363-2635041 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T213725 NZ_CP109835:c141281-141000 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT213725 WP_023289667.1 NZ_CP081893:c2636629-2636363 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT213725 NZ_CP109835:c141505-141278 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3