Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4612130..4612940 Replicon chromosome
Accession NZ_CP081892
Organism Klebsiella pneumoniae strain NY-06

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KZ657_RS22705 Protein ID WP_004178461.1
Coordinates 4612130..4612663 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KZ657_RS22710 Protein ID WP_002887278.1
Coordinates 4612674..4612940 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ657_RS22700 (4610961) 4610961..4612082 + 1122 WP_009309849.1 cupin domain-containing protein -
KZ657_RS22705 (4612130) 4612130..4612663 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KZ657_RS22710 (4612674) 4612674..4612940 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KZ657_RS22715 (4613043) 4613043..4614476 - 1434 WP_032415907.1 Cu(+)/Ag(+) sensor histidine kinase -
KZ657_RS22720 (4614466) 4614466..4615149 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KZ657_RS22725 (4615321) 4615321..4616706 + 1386 WP_047681547.1 efflux transporter outer membrane subunit -
KZ657_RS22730 (4616724) 4616724..4617068 + 345 WP_032433444.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T213711 WP_004178461.1 NZ_CP081892:c4612663-4612130 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213711 NZ_CP109825:42291-42776 [Serratia marcescens]
GTGGGATGTATAACCGCGCCAGTCCCGTTATCCAGCGATCACCAGCTGGCAGAGTTCGTCTGTGGAGAAGTTGTGCTCGA
TGACTGGCTTAAACAGAAAGGCTTAAAAAATCAGGCCCTCGGAGCTGCCCGAACGTTCGTTGTTTGTAAAGCAGAGACGA
AGCGAGTTCTCGGTTTTTACTCTCTGGCCACCGGCAGTGTTAACCATGTGGAAGCCACGGGGAACCTCCGACGTAATATG
CCAGATCCCATCCCCGTGATCATACTTGCGCGCCTGGCTGTCGATATCTCATTGCGTGGCAGAGGGCTTGGCGCAGATCT
ACTACACGATGCCGTGCTGCGCTGCTATCGGGTAGCTGAGAATATTGGCGTGCGCGCAATCATGGTTCATGCGCTATCAG
ATGAAGCGAAAGCTTTTTATATTCATCATGGCTTTAAGGTATCTCAGACACAGGAACGGACCCTGTTCCTCAGGGTGCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213711 WP_002887278.1 NZ_CP081892:c4612940-4612674 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213711 NZ_CP109825:42037-42303 [Serratia marcescens]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCGAAGGAATCTCAAAGGGCACTCATTGATGCTGCCGCGGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAAATGGCCTGTCAGGCTGCAGAGAACGTGATCCTCGACCGCCGTGTATTCAATT
TCAACGATGAACAGTATGCCGAGTTTATCGATATGCTTGACGCCCCCGTCACGGATGAGCCCGTAATTAACAAACTGCTG
ACAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References