Detailed information of TA system TA213699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1801058..1801648
• Replicon: chromosome
• Accession: NZ_CP081892
• Organism: Klebsiella pneumoniae strain NY-06

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KZ657_RS08730
• Protein ID: WP_021466317.1
• Coordinates: 1801316..1801648 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: KZ657_RS08725
• Protein ID: WP_000288812.1
• Coordinates: 1801058..1801315 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ657_RS08695 (1796329)	1796329..1797378	+	1050	WP_228740669.1	hypothetical protein	-
KZ657_RS08700 (1797396)	1797396..1798364	+	969	WP_000654811.1	IS5 family transposase	-
KZ657_RS08705 (1798357)	1798357..1799142	+	786	WP_228740670.1	hypothetical protein	-
KZ657_RS08710 (1799221)	1799221..1799778	+	558	WP_040245065.1	hypothetical protein	-
KZ657_RS08715 (1800061)	1800061..1800520	+	460	Protein_1706	hypothetical protein	-
KZ657_RS08720 (1800517)	1800517..1800723	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
KZ657_RS08725 (1801058)	1801058..1801315	+	258	WP_000288812.1	antitoxin	Antitoxin
KZ657_RS08730 (1801316)	1801316..1801648	+	333	WP_021466317.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KZ657_RS08740 (1801970)	1801970..1803406	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
KZ657_RS08750 (1803771)	1803771..1805225	-	1455	WP_004148975.1	AMP nucleosidase	-
KZ657_RS08755 (1805355)	1805355..1805600	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1797441..1798364	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.82 Da
• Isoelectric Point: 10.4722

>T213699 WP_021466317.1 NZ_CP081892:1801316-1801648 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDAVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T213699 NZ_CP109824:c14539-14159 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAGGGACGTTCT
GGTCGAGTATCACATCAATCCGGCCATCAGCGCCATCACCTGGATGGAAGTGATGGTAGGTGCTAAAAAACAGGGTCCGG
CACTGGAGCTGAAAACACGTCAGTTTTTAGGGCAATTTCTGCTGCTGCCTATTACCGATGAAGTCGCTGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAACTGCCAGACGCCATTATCTGGGCCACAGCGCAGGTGGGATTCCGGATGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTGATGCCTTATCGGCTGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT213699 WP_000288812.1 NZ_CP081892:1801058-1801315 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT213699 NZ_CP109824:c14760-14539 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGATAATCTGAAAAATATCCGCCACGTTCCGCG
GGCCGAGATCATTCGTCAAGCAGTCTCCCGCTATCTGACGGAGAACCACGTGGAAGATCCCAGCAACGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGAGTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0