Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5232217..5232842 Replicon chromosome
Accession NZ_CP081891
Organism Klebsiella pneumoniae strain CT-04

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KZ663_RS25360 Protein ID WP_002882817.1
Coordinates 5232217..5232600 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KZ663_RS25365 Protein ID WP_004150355.1
Coordinates 5232600..5232842 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ663_RS25345 (KZ663_25285) 5229583..5230485 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KZ663_RS25350 (KZ663_25290) 5230482..5231117 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KZ663_RS25355 (KZ663_25295) 5231114..5232043 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KZ663_RS25360 (KZ663_25300) 5232217..5232600 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ663_RS25365 (KZ663_25305) 5232600..5232842 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KZ663_RS25370 (KZ663_25310) 5233047..5233964 + 918 WP_087637200.1 alpha/beta hydrolase -
KZ663_RS25375 (KZ663_25315) 5233978..5234919 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KZ663_RS25380 (KZ663_25320) 5234964..5235401 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KZ663_RS25385 (KZ663_25325) 5235398..5236258 - 861 WP_004146232.1 virulence factor BrkB family protein -
KZ663_RS25390 (KZ663_25330) 5236252..5236851 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213694 WP_002882817.1 NZ_CP081891:c5232600-5232217 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213694 NZ_CP109802:c353764-353423 [Enterococcus hirae]
GTGCCTAAAGCTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGAAAT
TCAAAAGAGAAGACCAGGCTTGGTTGTATCTCGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTGTCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213694 WP_004150355.1 NZ_CP081891:c5232842-5232600 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213694 NZ_CP109802:c353994-353764 [Enterococcus hirae]
ATGCTCATTACAAAATCTAGATTACAAGGTAGTTCTGTAGTAATTACACTACCGTCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References