Detailed information of TA system TA213692

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4667158..4667861
• Replicon: chromosome
• Accession: NZ_CP081891
• Organism: Klebsiella pneumoniae strain CT-04

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KZ663_RS22675
• Protein ID: WP_040196446.1
• Coordinates: 4667158..4667499 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KZ663_RS22680
• Protein ID: WP_040196448.1
• Coordinates: 4667520..4667861 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ663_RS22650 (KZ663_22590)	4663735..4664862	+	1128	WP_023325431.1	PAAR domain-containing protein	-
KZ663_RS22655 (KZ663_22595)	4664882..4665115	+	234	WP_014908049.1	hypothetical protein	-
KZ663_RS22660 (KZ663_22600)	4665261..4665395	+	135	Protein_4441	transposase	-
KZ663_RS22665 (KZ663_22605)	4665408..4665518	-	111	Protein_4442	DUF4102 domain-containing protein	-
KZ663_RS22670 (KZ663_22610)	4665893..4666900	-	1008	WP_004216518.1	restriction endonuclease	-
KZ663_RS22675 (KZ663_22615)	4667158..4667499	-	342	WP_040196446.1	TA system toxin CbtA family protein	Toxin
KZ663_RS22680 (KZ663_22620)	4667520..4667861	-	342	WP_040196448.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KZ663_RS22685 (KZ663_22625)	4667872..4668414	-	543	WP_004894621.1	DNA repair protein RadC	-
KZ663_RS22690 (KZ663_22630)	4668427..4668870	-	444	WP_004894623.1	antirestriction protein	-
KZ663_RS22695 (KZ663_22635)	4668901..4669722	-	822	WP_040196452.1	DUF932 domain-containing protein	-
KZ663_RS22700 (KZ663_22640)	4669821..4670051	-	231	WP_014226751.1	DUF905 domain-containing protein	-
KZ663_RS22705 (KZ663_22645)	4670123..4670572	-	450	WP_004215773.1	IrmA family protein	-
KZ663_RS22710 (KZ663_22650)	4670569..4671021	-	453	WP_040196455.1	hypothetical protein	-
KZ663_RS22715 (KZ663_22655)	4671058..4671627	-	570	WP_040196457.1	hypothetical protein	-
KZ663_RS22720 (KZ663_22660)	4671627..4672331	-	705	WP_087637411.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4659608..4706901	47293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12791.74 Da
• Isoelectric Point: 7.1648

>T213692 WP_040196446.1 NZ_CP081891:c4667499-4667158 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T213692 NZ_CP109798:173813-174079 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12829.33 Da
• Isoelectric Point: 5.4792

>AT213692 WP_040196448.1 NZ_CP081891:c4667861-4667520 [Klebsiella pneumoniae]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLVDRASITGDFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAIYPTQR

Nucleotide

• Length: 342 bp

>AT213692 NZ_CP109798:173550-173813 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA