Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4631630..4632440 Replicon chromosome
Accession NZ_CP081891
Organism Klebsiella pneumoniae strain CT-04

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KZ663_RS22505 Protein ID WP_004178461.1
Coordinates 4631630..4632163 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KZ663_RS22510 Protein ID WP_002887278.1
Coordinates 4632174..4632440 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ663_RS22500 (KZ663_22440) 4630461..4631582 + 1122 WP_087637039.1 cupin domain-containing protein -
KZ663_RS22505 (KZ663_22445) 4631630..4632163 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KZ663_RS22510 (KZ663_22450) 4632174..4632440 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KZ663_RS22515 (KZ663_22455) 4632543..4633976 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
KZ663_RS22520 (KZ663_22460) 4633966..4634649 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KZ663_RS22525 (KZ663_22465) 4634821..4636206 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
KZ663_RS22530 (KZ663_22470) 4636224..4636568 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T213691 WP_004178461.1 NZ_CP081891:c4632163-4631630 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213691 NZ_CP109795:2015057-2015233 [Bacillus velezensis]
GTGTTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213691 WP_002887278.1 NZ_CP081891:c4632440-4632174 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213691 NZ_CP109795:c2015271-2015174 [Bacillus velezensis]
GAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAGCCG
CTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References