Detailed information of TA system TA213688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2668832..2670420
• Replicon: chromosome
• Accession: NZ_CP081891
• Organism: Klebsiella pneumoniae strain CT-04

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9J6RYF8
• Locus tag: KZ663_RS13150
• Protein ID: WP_004892016.1
• Coordinates: 2668832..2670154 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KZ663_RS13155
• Protein ID: WP_004176198.1
• Coordinates: 2670154..2670420 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ663_RS13135 (KZ663_13065)	2663888..2666389	+	2502	WP_004892009.1	fimbria/pilus outer membrane usher protein	-
KZ663_RS13140 (KZ663_13070)	2666377..2667381	+	1005	WP_004892012.1	fimbrial protein	-
KZ663_RS13145 (KZ663_13075)	2667523..2668815	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KZ663_RS13150 (KZ663_13080)	2668832..2670154	-	1323	WP_004892016.1	type II toxin-antitoxin system HipA family toxin	Toxin
KZ663_RS13155 (KZ663_13085)	2670154..2670420	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KZ663_RS13160 (KZ663_13090)	2670685..2671011	-	327	WP_004892019.1	hydroxyisourate hydrolase	-
KZ663_RS13165 (KZ663_13095)	2671008..2671508	-	501	WP_004892023.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KZ663_RS13170 (KZ663_13100)	2671486..2672865	-	1380	WP_004176204.1	nucleobase:cation symporter-2 family protein	-
KZ663_RS13175 (KZ663_13105)	2673211..2674365	+	1155	WP_004892025.1	FAD-dependent urate hydroxylase HpxO	-
KZ663_RS13180 (KZ663_13110)	2674346..2675269	-	924	WP_004892028.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2465476..2705903	240427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T213688 WP_004892016.1 NZ_CP081891:c2670154-2668832 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T213688 NZ_CP109795:533903-534253 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT213688 WP_004176198.1 NZ_CP081891:c2670420-2670154 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT213688 NZ_CP109795:533616-533897 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA