Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5135629..5136254 Replicon chromosome
Accession NZ_CP081890
Organism Klebsiella pneumoniae strain IA-021

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag KZ660_RS25085 Protein ID WP_004187928.1
Coordinates 5135629..5136012 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KZ660_RS25090 Protein ID WP_004150355.1
Coordinates 5136012..5136254 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ660_RS25070 (5132995) 5132995..5133897 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KZ660_RS25075 (5133894) 5133894..5134529 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KZ660_RS25080 (5134526) 5134526..5135455 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KZ660_RS25085 (5135629) 5135629..5136012 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ660_RS25090 (5136012) 5136012..5136254 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KZ660_RS25095 (5136448) 5136448..5137365 + 918 WP_004187929.1 alpha/beta hydrolase -
KZ660_RS25100 (5137379) 5137379..5138320 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KZ660_RS25105 (5138365) 5138365..5138802 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KZ660_RS25110 (5138799) 5138799..5139659 - 861 WP_004146232.1 virulence factor BrkB family protein -
KZ660_RS25115 (5139653) 5139653..5140252 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T213675 WP_004187928.1 NZ_CP081890:c5136012-5135629 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213675 NZ_CP109775:1636847-1637101 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213675 WP_004150355.1 NZ_CP081890:c5136254-5136012 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213675 NZ_CP109775:1636599-1636850 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References