Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5098552..5099177 Replicon chromosome
Accession NZ_CP081889
Organism Klebsiella pneumoniae strain IA-044

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KZ664_RS24780 Protein ID WP_002882817.1
Coordinates 5098552..5098935 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KZ664_RS24785 Protein ID WP_004150355.1
Coordinates 5098935..5099177 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ664_RS24765 (KZ664_24720) 5095918..5096820 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KZ664_RS24770 (KZ664_24725) 5096817..5097452 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KZ664_RS24775 (KZ664_24730) 5097449..5098378 + 930 WP_046654801.1 formate dehydrogenase accessory protein FdhE -
KZ664_RS24780 (KZ664_24735) 5098552..5098935 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KZ664_RS24785 (KZ664_24740) 5098935..5099177 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KZ664_RS24790 (KZ664_24745) 5099382..5100299 + 918 WP_004181612.1 alpha/beta hydrolase -
KZ664_RS24795 (KZ664_24750) 5100313..5101254 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KZ664_RS24800 (KZ664_24755) 5101299..5101736 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KZ664_RS24805 (KZ664_24760) 5101733..5102593 - 861 WP_046654802.1 virulence factor BrkB family protein -
KZ664_RS24810 (KZ664_24765) 5102587..5103186 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213656 WP_002882817.1 NZ_CP081889:c5098935-5098552 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213656 NZ_CP109761:2744145-2744840 [Priestia megaterium]
TTGTATGGAGAAGAATTGAGTCTTTTGTTGGAAAGCAAACAGCAAAATGTCAGTTATGATATAGGACGATTATGCGGGGT
TATATATGAGATCATCGATCTTCATAAGGAGTATACACAACAAAATATTAACAGTTATAACTTGAAAAAAACTTCGGTTA
ATCATATTTTGGAACAAGTTGATGATACTGATACATATATTATTAACAAGAACTTTCATACAACACTAAATAAATATCTG
AGTCATGTGGATAGGTTGACTGTTGATTGTGAGTTAGACTACACTTATAGCGACTTAGATTGTCGAGTTAGAGTTAAGCA
ACAAGAGTCCATTGTCTATAAAATTGGTCATTACCATAGTGGTAAATCAGAGGGCGGAAGGTTACCCTTAAACAAATGTT
TAAATGATTTGGTAGGGTTTCGGATAGTCGTACCAAACTTTAATCATAGTTGCCAGTTATTTGAGGAAATGTGTGGATAT
ATAGGAGAAAATTATAGAATTAAGTATAGAGATTCTTCAAAAGGGGAATATAAGGCTACACACATATATTTCTATGGAGA
AGGTAATAAGAATTACCCGTGGGAATTACAAATATGGTTACCAGAAGATCACGCAAATAATTATGAATCCCATAATAGAC
ATAAACAAGGGTATAAGGAGTCTGCAAAAATCCACAAAGAAAGTTTTACATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213656 WP_004150355.1 NZ_CP081889:c5099177-5098935 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213656 NZ_CP109761:2744854-2745603 [Priestia megaterium]
ATGGCATTTCATTTTATAGCAATTATGAGTCATTATATTGAGGGGTCAAGAATCGGTTGGCACTACTCCAGTGAGGGAAG
ACTTGACAAAGAAGTCATTAACTCTTTCTTTAGTAGAGTAAAGAAAAAGTGTGGGGAGGTTCATTTTGGTATCCATAAAC
TATCCACAGACTCTACTTCATGGAAATCTGTAGTAGAGAAAGACTCGTTTTTTGATGATGTGATTTCTACAGAGGATATG
GATATGTTTATTGATTATGTATCAAGTGATCAACAATTATCTGCACGCGATGTAGCAAAATTTATATTATCATCTATTCC
AGTTTCACACCTTAAATTGCAAAAGCTTCTTTATTATTGCTATGCAGAATTCATTCAAAGAACAGGTTTAAAATTGTTTA
GAGAGCCGATTGTTTCATATAAATATGGTCCTGTTGTTGAAAGTATATTTAAAGAATTTACTGTTCATGGATCATCAATA
ATTGATTACGAAGAGGATAAAGGTTTTGTAATCTCTGTAGATAAAATGACGGCAAAGCCCTCTTTTATGAAGATTGCTAA
TTCAGAACACGGTGTAGTAGCTCTTGACTGTATTATAGATGTTTTAGAGCAGTATGGGTCCAAGTCACCTTTTGAATTAG
TTGATATTACACATCAAGAAGGTGGACCCTGGGCCAGAGTTTATAAACCAGGACAAAACAACATTATAACTGACGACCAC
ATAATACAGTATCATGAATTCGCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References