Detailed information of TA system TA213654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4516519..4517329
• Replicon: chromosome
• Accession: NZ_CP081889
• Organism: Klebsiella pneumoniae strain IA-044

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KZ664_RS22070
• Protein ID: WP_048337996.1
• Coordinates: 4516519..4517052 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: KZ664_RS22075
• Protein ID: WP_002887278.1
• Coordinates: 4517063..4517329 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ664_RS22065 (KZ664_22015)	4515350..4516471	+	1122	WP_004214138.1	cupin domain-containing protein	-
KZ664_RS22070 (KZ664_22020)	4516519..4517052	-	534	WP_048337996.1	type II toxin-antitoxin system toxin KacT	Toxin
KZ664_RS22075 (KZ664_22025)	4517063..4517329	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
KZ664_RS22080 (KZ664_22030)	4517432..4518865	-	1434	WP_048291165.1	Cu(+)/Ag(+) sensor histidine kinase	-
KZ664_RS22085 (KZ664_22035)	4518855..4519538	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
KZ664_RS22090 (KZ664_22040)	4519710..4521095	+	1386	WP_047694163.1	efflux transporter outer membrane subunit	-
KZ664_RS22095 (KZ664_22045)	4521113..4521457	+	345	WP_094833307.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19824.68 Da
• Isoelectric Point: 5.2614

>T213654 WP_048337996.1 NZ_CP081889:c4517052-4516519 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSLQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T213654 NZ_CP109758:481688-482029 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT213654 WP_002887278.1 NZ_CP081889:c4517329-4517063 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT213654 NZ_CP109758:481458-481688 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1