Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3922319..3922938 Replicon chromosome
Accession NZ_CP081889
Organism Klebsiella pneumoniae strain IA-044

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KZ664_RS19210 Protein ID WP_002892050.1
Coordinates 3922720..3922938 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KZ664_RS19205 Protein ID WP_002892066.1
Coordinates 3922319..3922693 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ664_RS19195 (KZ664_19160) 3917471..3918664 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KZ664_RS19200 (KZ664_19165) 3918687..3921833 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KZ664_RS19205 (KZ664_19170) 3922319..3922693 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KZ664_RS19210 (KZ664_19175) 3922720..3922938 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KZ664_RS19215 (KZ664_19180) 3923097..3923663 + 567 WP_002892030.1 maltose O-acetyltransferase -
KZ664_RS19220 (KZ664_19185) 3923635..3923775 - 141 WP_004147370.1 hypothetical protein -
KZ664_RS19225 (KZ664_19190) 3923796..3924266 + 471 WP_048337480.1 YlaC family protein -
KZ664_RS19230 (KZ664_19195) 3924241..3925692 - 1452 WP_048337479.1 PLP-dependent aminotransferase family protein -
KZ664_RS19235 (KZ664_19200) 3925793..3926491 + 699 WP_002892021.1 GNAT family protein -
KZ664_RS19240 (KZ664_19205) 3926488..3926628 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KZ664_RS19245 (KZ664_19210) 3926628..3926891 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213653 WP_002892050.1 NZ_CP081889:3922720-3922938 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T213653 NZ_CP109758:190267-190533 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT213653 WP_002892066.1 NZ_CP081889:3922319-3922693 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT213653 NZ_CP109758:190004-190267 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGTTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References