Detailed information of TA system TA021365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2106629..2107143
• Replicon: chromosome
• Accession: NC_007622
• Organism: Staphylococcus aureus RF122

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAB_RS10680
• Protein ID: WP_000581792.1
• Coordinates: 2106629..2106838 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: SAB_RS10685
• Protein ID: WP_000138929.1
• Coordinates: 2106850..2107143 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAB_RS10660	2102137..2103495	-	1359	WP_000611485.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAB_RS10665	2103510..2104580	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SAB_RS10670	2104898..2106100	+	1203	WP_001109943.1	rod shape-determining protein RodA	-
SAB_RS10675	2106364..2106501	-	138	WP_000828354.1	hypothetical protein	-
SAB_RS10680	2106629..2106838	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAB_RS10685	2106850..2107143	-	294	WP_000138929.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAB_RS10690	2107309..2108793	+	1485	WP_000571547.1	cardiolipin synthase	-
SAB_RS10695	2108821..2109468	+	648	WP_001187616.1	HD domain-containing protein	-
SAB_RS10700	2110132..2111004	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAB_RS10705	2111090..2111731	-	642	WP_000483166.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T21365 WP_000581792.1 NC_007622:c2106838-2106629 [Staphylococcus aureus RF122]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T21365 NC_007622:c2106838-2106629 [Staphylococcus aureus RF122]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11215.86 Da
• Isoelectric Point: 6.2243

>AT21365 WP_000138929.1 NC_007622:c2107143-2106850 [Staphylococcus aureus RF122]
MTEQDNAHHSEQIKMNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT21365 NC_007622:c2107143-2106850 [Staphylococcus aureus RF122]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAATGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGCGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1