Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39998..40523 Replicon plasmid unnamed2
Accession NZ_CP081887
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag K5O11_RS24110 Protein ID WP_001159871.1
Coordinates 40218..40523 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag K5O11_RS24105 Protein ID WP_000813630.1
Coordinates 39998..40216 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS24080 (35208) 35208..35894 + 687 Protein_37 IS1 family transposase -
K5O11_RS24085 (36148) 36148..37170 - 1023 WP_000361402.1 helicase UvrD -
K5O11_RS24090 (37155) 37155..38720 - 1566 WP_001128474.1 AAA family ATPase -
K5O11_RS24095 (38795) 38795..39211 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
K5O11_RS24100 (39208) 39208..39438 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
K5O11_RS24105 (39998) 39998..40216 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K5O11_RS24110 (40218) 40218..40523 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
K5O11_RS24115 (40524) 40524..41330 + 807 WP_000016970.1 site-specific integrase -
K5O11_RS24120 (42052) 42052..42807 + 756 WP_000852146.1 replication initiation protein RepE -
K5O11_RS24125 (43395) 43395..44561 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sitABCD / blaTEM-1B / sul3 / tet(A) / sul2 / floR iucA / iucB / iucC / iucD / iutA 1..114381 114381
- flank IS/Tn - - 35547..35894 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T213630 WP_001159871.1 NZ_CP081887:40218-40523 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T213630 NZ_CP109744:1324548-1324859 [Citrobacter portucalensis]
ATGCTATTCATAGAAACGGAAATCTTCACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGATTACAGAT
ATTCCTTGCGATTCAGCCTGATTGTGGCGATCTGATACAAGAAACGGGCGGACTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGCAAACGTAGCGGTGTGAGGATTATTTACTTTCATCAAATCAGAAAATCACAAATTCGATTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAAGAAAAAGTGCTGCTGCGTATGTTGAATGAGAGGTGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT213630 WP_000813630.1 NZ_CP081887:39998-40216 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT213630 NZ_CP109744:1324860-1325147 [Citrobacter portucalensis]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGGTTCAGATGAATGAGATCGCTGAAGGAACGCGACAACCTTC
CCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATTCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAAGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCTATTGCCAATGATCCAAAACATGTCATTCAGGCACTAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References