Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38795..39438 Replicon plasmid unnamed2
Accession NZ_CP081887
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K5O11_RS24095 Protein ID WP_001034044.1
Coordinates 38795..39211 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K5O11_RS24100 Protein ID WP_001261286.1
Coordinates 39208..39438 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS24080 (35208) 35208..35894 + 687 Protein_37 IS1 family transposase -
K5O11_RS24085 (36148) 36148..37170 - 1023 WP_000361402.1 helicase UvrD -
K5O11_RS24090 (37155) 37155..38720 - 1566 WP_001128474.1 AAA family ATPase -
K5O11_RS24095 (38795) 38795..39211 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O11_RS24100 (39208) 39208..39438 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5O11_RS24105 (39998) 39998..40216 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
K5O11_RS24110 (40218) 40218..40523 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
K5O11_RS24115 (40524) 40524..41330 + 807 WP_000016970.1 site-specific integrase -
K5O11_RS24120 (42052) 42052..42807 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sitABCD / blaTEM-1B / sul3 / tet(A) / sul2 / floR iucA / iucB / iucC / iucD / iutA 1..114381 114381
- flank IS/Tn - - 35547..35894 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T213629 WP_001034044.1 NZ_CP081887:c39211-38795 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T213629 NZ_CP109744:c1323314-1323003 [Citrobacter portucalensis]
TTGCATGTTATCAGCCGAAAACCTTTTAACGAGGCAATGCTCATGTATCCAAATCATCAGCTTGCTCTCGCTGATTTACT
GAATGTTTTGGAGAAAAAAACATTCATTCAGCCTGAAGAGATGAAGCAGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTAAGACTCATCTCCTACATAGATTTCAGGTCACATAAGATA
TTTGTCAAACATATCGTTTCCCATGCCGAATATGACAAACTGACAGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT213629 WP_001261286.1 NZ_CP081887:c39438-39208 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT213629 NZ_CP109744:c1323006-1322560 [Citrobacter portucalensis]
ATGAGAACGCATCGTCATATGGATACAGCCAGCGCAAAAAAGATTGTTGATACCTTCAGCGATGCGGTAAAAACCATCCC
ACTGTTGGGGGAAGACCGAAATGACACTGAGTATCGCAGGGCACTTGCGCTAGTGGAGTATCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTAAATATGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACACCCTCTGGCGTTTCAGTATTGCGTACGCTCATGGACCAATACAGTCTCAAAGCATCAGACCT
TGCCAACGAACTCGGTTCAAAATCGAACGTCAGCAACATCTTAAATGGTCGCAGAGCACTGACTGTAAGCCATATTAAAG
CGCTTGCAGAACGCTTTAATCTTCCGGCAGACGCATTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References