Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4694711..4695327 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag K5O11_RS22405 Protein ID WP_001129490.1
Coordinates 4694711..4695085 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag K5O11_RS22410 Protein ID WP_001275523.1
Coordinates 4695085..4695327 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS22390 (4692214) 4692214..4693116 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
K5O11_RS22395 (4693113) 4693113..4693748 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K5O11_RS22400 (4693745) 4693745..4694674 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
K5O11_RS22405 (4694711) 4694711..4695085 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O11_RS22410 (4695085) 4695085..4695327 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
K5O11_RS22415 (4695547) 4695547..4695765 - 219 WP_001251290.1 CopG family transcriptional regulator -
K5O11_RS25345 (4696439) 4696439..4697353 - 915 WP_072649161.1 transposase -
K5O11_RS25350 (4697366) 4697366..4698253 - 888 Protein_4409 hypothetical protein -
K5O11_RS22425 (4698669) 4698669..4699610 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
K5O11_RS22430 (4699655) 4699655..4700092 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T213628 WP_001129490.1 NZ_CP081881:c4695085-4694711 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T213628 NZ_CP109744:1079139-1079480 [Citrobacter portucalensis]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGACCGTTGCAGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAGCAACACTATGGCCTGACGCTGAACGACACACCATTCAGTGATGTAACCGTTATTCAGGAGCATATCA
ACGCCGGTATTACGCTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAAGTTTT
AACTGTCAGGAGCAATCACCTTATATTCGGGCGGTAGATATTTTACGGGCAAGACAGGCAACTGGCCTGTTGCGACAAAG
CCGTAACAACGCAGTACGGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT213628 WP_001275523.1 NZ_CP081881:c4695327-4695085 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT213628 NZ_CP109744:1078800-1079117 [Citrobacter portucalensis]
ATGAGCAACACCACATGGGGCCTGCAGCGGGATATTACACCGCGCATGGGGGCACGTCTGGTACAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCAGTAAGTTTAGTGATGCCGAATGCCAGAAGTTGGATATCGCATTTCCGC
ATTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGGCATGCCCATTGCGTCACCCTGTACCAC
AACGGTTATACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCTGTTTATCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References