Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4170975..4171551 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag K5O11_RS19980 Protein ID WP_001131965.1
Coordinates 4171264..4171551 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag K5O11_RS19975 Protein ID WP_000063158.1
Coordinates 4170975..4171277 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS19955 (4166083) 4166083..4167303 + 1221 WP_000873756.1 amino acid deaminase -
K5O11_RS19960 (4167571) 4167571..4169721 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
K5O11_RS19965 (4169771) 4169771..4169974 + 204 WP_000467859.1 YbdD/YjiX family protein -
K5O11_RS19970 (4169985) 4169985..4170941 + 957 WP_001361361.1 GTPase -
K5O11_RS19975 (4170975) 4170975..4171277 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
K5O11_RS19980 (4171264) 4171264..4171551 - 288 WP_001131965.1 BrnT family toxin Toxin
K5O11_RS19985 (4171748) 4171748..4172914 + 1167 WP_000800823.1 restriction endonuclease -
K5O11_RS19990 (4172959) 4172959..4173132 + 174 Protein_3939 type I restriction-modification system subunit M N-terminal domain-containing protein -
K5O11_RS19995 (4173126) 4173126..4173794 + 669 Protein_3940 DUF3387 domain-containing protein -
K5O11_RS20000 (4174009) 4174009..4174347 + 339 WP_000132633.1 endoribonuclease SymE -
K5O11_RS25335 (4174435) 4174435..4174482 - 48 WP_229017048.1 hypothetical protein -
K5O11_RS20010 (4174730) 4174730..4176142 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T213624 WP_001131965.1 NZ_CP081881:c4171551-4171264 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T213624 NZ_CP109739:3000577-3000933 [Serratia nevei]
ATGATCTTTTTGACCGCAGAGGATATTGCGGAATTCAATGCTGAAATAGTGCCTCACGGCCGCCAGGAAGGCAGAAAAGT
TGAGGCTGCCGCCAACCGCGTGCTCAATGCTTACCACTACGAAAACGTCACCGACGTTTATCGTTTGGCCGCGCTCTATC
TCATCGCCATCAGCCACGGTCACATTTTTCTTGATGGCAACAAACGTACGGCCTTCCAAAGCATGGCGCTATTTCTCGGT
ATTAATGGCATTGCACTGCGGGAAGATGCAGAATTGGTCGAAATTACGGTTGAAGCAGCTCAAGGCCGCCTCAACGTTGA
ACAAGCCGCTGAACACTTACGCCTGCTAACCGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT213624 WP_000063158.1 NZ_CP081881:c4171277-4170975 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT213624 NZ_CP109739:3000371-3000580 [Serratia nevei]
ATGACTACGATTAGCTACACGGCGGCGAGAAACAATTTGGCCGAGGTGCTTTTGGAAGCGCAAAAACAGCCGGTGGAAGT
GACGCGCCGCGGACACGATGAGGTTTACATCATCAGTAAGGCCGACTATGAGGTCTTGGTCAAAGCCAAGGTGAAAGCGC
GCATTCAGGCTAAACACGCGGATACGATTAAGGCGCTGGCGAATAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References