Detailed information of TA system TA213623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3675671..3676508
• Replicon: chromosome
• Accession: NZ_CP081881
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: K5O11_RS17625
• Protein ID: WP_000227782.1
• Coordinates: 3675966..3676508 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: K5O11_RS17620
• Protein ID: WP_001353405.1
• Coordinates: 3675671..3675982 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS17595 (3670691)	3670691..3671638	+	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-
K5O11_RS17600 (3671660)	3671660..3673651	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K5O11_RS17605 (3673641)	3673641..3674255	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K5O11_RS17610 (3674255)	3674255..3674584	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K5O11_RS17615 (3674596)	3674596..3675486	+	891	WP_000971327.1	heme o synthase	-
K5O11_RS17620 (3675671)	3675671..3675982	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
K5O11_RS17625 (3675966)	3675966..3676508	+	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
K5O11_RS17630 (3676564)	3676564..3677408	-	845	Protein_3473	sel1 repeat family protein	-
K5O11_RS17635 (3677815)	3677815..3679179	+	1365	WP_001000978.1	MFS transporter	-
K5O11_RS17640 (3679307)	3679307..3679798	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K5O11_RS17645 (3679966)	3679966..3680877	+	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
K5O11_RS17650 (3680840)	3680840..3681430	+	591	WP_001276320.1	protein deglycase YajL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T213623 WP_000227782.1 NZ_CP081881:3675966-3676508 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T213623 NZ_CP109739:2920044-2920433 [Serratia nevei]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAAGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCCGCGGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGCTGCTACGGTGAAATG
CGCGCGAACATGGAGAGAAAGGGCAAAATCATGGGCGCGCTGGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT213623 WP_001353405.1 NZ_CP081881:3675671-3675982 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT213623 NZ_CP109739:2919777-2920040 [Serratia nevei]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAGTTTCTTGGCGCGGAAGATCGTCAGCAAGGGGTTGCAGACCGC
GATCCATTCTCAGAGGAAGAGTAG

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3