Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3641581..3642199 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K5O11_RS17455 Protein ID WP_001291435.1
Coordinates 3641981..3642199 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K5O11_RS17450 Protein ID WP_000344800.1
Coordinates 3641581..3641955 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS17440 (3636670) 3636670..3637863 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5O11_RS17445 (3637886) 3637886..3641035 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K5O11_RS17450 (3641581) 3641581..3641955 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K5O11_RS17455 (3641981) 3641981..3642199 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K5O11_RS17460 (3642371) 3642371..3642922 + 552 WP_000102574.1 maltose O-acetyltransferase -
K5O11_RS17465 (3643038) 3643038..3643508 + 471 WP_000136192.1 YlaC family protein -
K5O11_RS17470 (3643672) 3643672..3645222 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K5O11_RS17475 (3645264) 3645264..3645617 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K5O11_RS17485 (3645996) 3645996..3646307 + 312 WP_000409911.1 MGMT family protein -
K5O11_RS17490 (3646338) 3646338..3646910 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213622 WP_001291435.1 NZ_CP081881:3641981-3642199 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213622 NZ_CP109739:2734165-2734440 [Serratia nevei]
ATGAAAGAGCAGGTTTCAGCGTTAAGGAAGCGACAAAAAAGCACATTAGAACAGATATTTAAAACGCCTGTTCTTTCCGG
GATCAAATGGTCAGATGTGGAATCACTGATAAAAGCGCTGGGTGGGGAAGTTAAAGAGGGGCGGGGATCGCGGTGTAAGT
TTCTGCTCAATGGCAGCATCGCCAATTTTCACCGGCCACACCCATCACCAGACACGGACAAGGGCGCGGTAGTCAGTCTC
CGCGAATGGCTGGAAAGCATAGGAGTTAAGCCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213622 WP_000344800.1 NZ_CP081881:3641581-3641955 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213622 NZ_CP109739:2734437-2734802 [Serratia nevei]
ATGACGAAAACAGCAGCAGTAACCCCGAACACTATGGAGATCGCCGGGCAGCCGGCGATCATCAGCTACGTACCAGAGAT
CGGCGCGTTTCGCGGTAAGTTCCTCGGGCTGTCCGGTTACTGTGATTTCGTTTCGGACAGCATTCAGGGGCTGAAAGCTG
AAGGGGAGATCTCTTTGCGCGAATACCTGGACGATTGCAGCGCCGCAGGGATAGAACCATACGCCCGGCAAGAGAAAATC
AAGACGTTTACTCTGCGCTATCCGGAGTCTTTCGGGGAACGCCTGAACCAGGCGGCGGCAGAAAATGAGGTCTCCGTAAA
TGCCTTTATTGTCGAAACATTGAATGAGCGAATGAAACACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References