Detailed information of TA system TA213620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3216659..3217364
• Replicon: chromosome
• Accession: NZ_CP081881
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: K5O11_RS15360
• Protein ID: WP_000539524.1
• Coordinates: 3216659..3217045 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K5O11_RS15365
• Protein ID: WP_001280945.1
• Coordinates: 3217035..3217364 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS15340 (3212663)	3212663..3213289	+	627	WP_001295292.1	glutathione S-transferase GstB	-
K5O11_RS15345 (3213286)	3213286..3214401	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
K5O11_RS15350 (3214512)	3214512..3214895	-	384	WP_000497137.1	biofilm formation regulator BssR	-
K5O11_RS15355 (3215108)	3215108..3216433	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
K5O11_RS15360 (3216659)	3216659..3217045	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5O11_RS15365 (3217035)	3217035..3217364	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
K5O11_RS15370 (3217434)	3217434..3218751	-	1318	Protein_3034	diguanylate cyclase	-
K5O11_RS15375 (3218759)	3218759..3221107	-	2349	WP_000950331.1	EAL domain-containing protein	-
K5O11_RS15380 (3221284)	3221284..3222195	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T213620 WP_000539524.1 NZ_CP081881:3216659-3217045 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T213620 NZ_CP109739:1333713-1334093 [Serratia nevei]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAATTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT213620 WP_001280945.1 NZ_CP081881:3217035-3217364 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT213620 NZ_CP109739:1333492-1333713 [Serratia nevei]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGGCGGAGAACCACGTTGAAGATCCCAGCAATGCCTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7