Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 903980..904634 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag K5O11_RS04415 Protein ID WP_000244781.1
Coordinates 904227..904634 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K5O11_RS04410 Protein ID WP_000354046.1
Coordinates 903980..904246 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS04390 (900068) 900068..901501 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
K5O11_RS04395 (901546) 901546..901857 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
K5O11_RS04400 (902021) 902021..902680 + 660 WP_000250269.1 hemolysin III family protein -
K5O11_RS04405 (902757) 902757..903737 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
K5O11_RS04410 (903980) 903980..904246 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K5O11_RS04415 (904227) 904227..904634 + 408 WP_000244781.1 protein YgfX Toxin
K5O11_RS04420 (904674) 904674..905195 - 522 WP_001521233.1 flavodoxin FldB -
K5O11_RS04425 (905307) 905307..906203 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K5O11_RS04430 (906228) 906228..906938 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K5O11_RS04435 (906944) 906944..908677 + 1734 WP_063117505.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T213606 WP_000244781.1 NZ_CP081881:904227-904634 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T213606 NZ_CP109727:2324184-2324705 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGGCTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGCCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT213606 WP_000354046.1 NZ_CP081881:903980-904246 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT213606 NZ_CP109727:2323908-2324177 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References