Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 352310..352941 Replicon chromosome
Accession NZ_CP081881
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag K5O11_RS01595 Protein ID WP_001260301.1
Coordinates 352310..352585 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag K5O11_RS01600 Protein ID WP_000593555.1
Coordinates 352582..352941 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS01580 (347314) 347314..348381 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
K5O11_RS01585 (348378) 348378..351113 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
K5O11_RS01590 (351113) 351113..352237 + 1125 WP_001314210.1 ABC transporter permease -
K5O11_RS01595 (352310) 352310..352585 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
K5O11_RS01600 (352582) 352582..352941 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K5O11_RS01605 (353061) 353061..353462 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
K5O11_RS01610 (353467) 353467..354273 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
K5O11_RS01615 (354270) 354270..355034 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
K5O11_RS01620 (355034) 355034..355867 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
K5O11_RS01625 (355864) 355864..356808 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 345326..353462 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T213603 WP_001260301.1 NZ_CP081881:352310-352585 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T213603 NZ_CP109727:1167530-1167817 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTTGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT213603 WP_000593555.1 NZ_CP081881:352582-352941 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT213603 NZ_CP109727:1167804-1168106 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGAGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGAAGCAGAGATTGATTACAGCGATATCCCTGCTACGGACGATGAACAGTGGTCAGGTGCGGTGCGAGGAAAGT
TTTATCGTCCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAAAGGCCAGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References