Detailed information of TA system TA213601

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 70767..71565
• Replicon: chromosome
• Accession: NZ_CP081881
• Organism: Escherichia coli strain WW2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K5O11_RS00305
• Protein ID: WP_061092894.1
• Coordinates: 70767..71144 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K5O11_RS00310
• Protein ID: WP_061092893.1
• Coordinates: 71191..71565 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O11_RS00275 (66130)	66130..67053	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
K5O11_RS00280 (67164)	67164..68348	-	1185	WP_001172864.1	sugar efflux transporter	-
K5O11_RS00285 (68747)	68747..68908	-	162	Protein_56	virulence RhuM family protein	-
K5O11_RS00290 (69146)	69146..69988	-	843	WP_061092922.1	DUF4942 domain-containing protein	-
K5O11_RS00295 (70073)	70073..70270	-	198	WP_012908799.1	DUF957 domain-containing protein	-
K5O11_RS00300 (70282)	70282..70770	-	489	WP_061092895.1	DUF5983 family protein	-
K5O11_RS00305 (70767)	70767..71144	-	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
K5O11_RS00310 (71191)	71191..71565	-	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K5O11_RS00315 (71728)	71728..71949	-	222	WP_061092892.1	DUF987 domain-containing protein	-
K5O11_RS00320 (72012)	72012..72488	-	477	WP_001186188.1	RadC family protein	-
K5O11_RS00325 (72503)	72503..72982	-	480	WP_000706978.1	antirestriction protein	-
K5O11_RS00330 (73248)	73248..74066	-	819	WP_001175165.1	DUF932 domain-containing protein	-
K5O11_RS00335 (74156)	74156..74389	-	234	WP_001278293.1	DUF905 family protein	-
K5O11_RS00340 (74395)	74395..74703	-	309	Protein_67	hypothetical protein	-
K5O11_RS00345 (74771)	74771..75948	+	1178	WP_152895938.1	IS3 family transposase	-
K5O11_RS00350 (75956)	75956..76333	-	378	Protein_69	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	59421..86389	26968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T213601 WP_061092894.1 NZ_CP081881:c71144-70767 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T213601 NZ_CP109727:c836635-836348 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT213601 WP_061092893.1 NZ_CP081881:c71565-71191 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT213601 NZ_CP109727:c836944-836642 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA