Detailed information of TA system TA213600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 176613..177103
• Replicon: plasmid p1-UTDF19-27C
• Accession: NZ_CP081879
• Organism: Exiguobacterium acetylicum strain UTDF19-27C

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6T22_RS17245
• Protein ID: WP_238240344.1
• Coordinates: 176834..177103 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A031ILR5
• Locus tag: K6T22_RS17240
• Protein ID: WP_026829710.1
• Coordinates: 176613..176837 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T22_RS17225 (K6T22_17220)	172868..174241	-	1374	WP_238240338.1	diguanylate cyclase	-
K6T22_RS17320	174620..174754	-	135	WP_283205713.1	hypothetical protein	-
K6T22_RS17230 (K6T22_17225)	174870..175862	+	993	WP_238240340.1	DNA/RNA non-specific endonuclease	-
K6T22_RS17235 (K6T22_17230)	176088..176378	+	291	WP_238240342.1	YolD-like family protein	-
K6T22_RS17240 (K6T22_17235)	176613..176837	+	225	WP_026829710.1	DUF6290 family protein	Antitoxin
K6T22_RS17245 (K6T22_17240)	176834..177103	+	270	WP_238240344.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6T22_RS17250 (K6T22_17245)	177335..179341	-	2007	WP_238240347.1	heavy metal translocating P-type ATPase	-
K6T22_RS17255 (K6T22_17250)	179774..180277	+	504	WP_050678841.1	PCYCGC motif-containing (lipo)protein	-
K6T22_RS17260 (K6T22_17255)	180315..180995	-	681	WP_047394277.1	response regulator transcription factor	-
K6T22_RS17265 (K6T22_17260)	181318..181644	-	327	WP_071908772.1	four-helix bundle copper-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..184468	184468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-88)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10560.25 Da
• Isoelectric Point: 10.5054

>T213600 WP_238240344.1 NZ_CP081879:176834-177103 [Exiguobacterium acetylicum]
MTTYKVEFERGAQKSLKKMDPQQTRIIMSWIKKNLVGTDDPRRHGKGLVSNRSGEWRYRIGDYRLIADIQDEKVVILILE
IGHRRDIYK

Nucleotide

• Length: 270 bp

>T213600 NZ_CP109727:c654299-653946 [Enterobacter roggenkampii]
GTGGACGGTTCGGTTCGGTTCGGAAAGGTATTCGAGCAGTGGTTGTTTGAACAAGAAGAAGGATTACAGGATAAGGTACT
GGCAGACCTGCTAAATCTGCAACACTATGGGCCACGTCTTCCTCGTCCCTATGCAGATACGGTTAAAGGCTCACGGTATA
AGCATATGAAAGAATTACGTATTCAATATGCTGGTCGTCCTGTTCGTGCTTTCTTCGCATTTGATCCTGTACGTCAGGCC
ATTGTGCTTTGCGCGGGCGATAAGAGTAACGACAAAACCTTTTATGAAAAAATGATTCGCATCGCAGATGCCGAATTTTC
CCTTCATCTGACAAGCCAAGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8599.76 Da
• Isoelectric Point: 4.5010

>AT213600 WP_026829710.1 NZ_CP081879:176613-176837 [Exiguobacterium acetylicum]
MSTISVRLDDQDTRLIKEYAKAKNITISTLVRDAVLDRIEDEIDLQLYHDSMAAHRKKSEAISFDDMMKELDLE

Nucleotide

• Length: 225 bp

>AT213600 NZ_CP109727:c653949-653650 [Enterobacter roggenkampii]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGTTCACCGGAAAGCCAACAACGTATTAAAGAGATGGCAGAAGAAATGAT
CCTCGAAACGGGTCTGCAACTTTTACGCGAAGAGCTCAGGCTTTCGCAAAAAAAAGTTGCTGACCTGATGGGGGTGAGCC
AGCCCGCAGTAACTCAGATAGAGCAACGTGGAAATGAACTAAAAATTGCCACGCTTAAGCGTTACATCGAGGCAATGGGT
GGGAAACTCTGTATCGACGTTGAACTTCCCACAGGAAAACGCGTTGCATTTCATATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A031ILR5