Detailed information of TA system TA213597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2359621..2360599
• Replicon: chromosome
• Accession: NZ_CP081872
• Organism: Bacillus toyonensis strain UTDF19-29B

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: K0FNW8
• Locus tag: K6T24_RS11945
• Protein ID: WP_000155917.1
• Coordinates: 2359621..2360361 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: J8KE32
• Locus tag: K6T24_RS11950
• Protein ID: WP_000588713.1
• Coordinates: 2360474..2360599 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T24_RS11925 (K6T24_11860)	2355284..2356066	+	783	WP_000381789.1	class I SAM-dependent methyltransferase	-
K6T24_RS11930 (K6T24_11865)	2356245..2357921	-	1677	WP_000857135.1	alpha-keto acid decarboxylase family protein	-
K6T24_RS11935 (K6T24_11870)	2358048..2358530	+	483	WP_000196695.1	MarR family winged helix-turn-helix transcriptional regulator	-
K6T24_RS11940 (K6T24_11875)	2358697..2359434	+	738	WP_000594129.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
K6T24_RS11945 (K6T24_11880)	2359621..2360361	+	741	WP_000155917.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K6T24_RS11950 (K6T24_11885)	2360474..2360599	+	126	WP_000588713.1	hypothetical protein	Antitoxin
K6T24_RS11955 (K6T24_11890)	2360675..2360851	+	177	WP_085450779.1	stage II sporulation protein SB	-
K6T24_RS11960 (K6T24_11895)	2360900..2361288	-	389	Protein_2273	YxeA family protein	-
K6T24_RS11965 (K6T24_11900)	2361532..2363001	+	1470	WP_000287539.1	beta-Ala-His dipeptidase	-
K6T24_RS11970 (K6T24_11905)	2363247..2364056	+	810	WP_000678367.1	papain-like cysteine protease family protein	-
K6T24_RS11975 (K6T24_11910)	2364082..2364684	+	603	WP_000517273.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28446.03 Da
• Isoelectric Point: 7.3946

>T213597 WP_000155917.1 NZ_CP081872:2359621-2360361 [Bacillus toyonensis]
MTISNIRIGLFILAIVFVVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIS
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTNIIQNINPAAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAANELNTGFRGIRSQFSITIPIEHIEQLNEQKAVQVENVGIIPAKIMNDVFIVIDGKKNSLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T213597 NZ_CP109697:c40971-40486 [Enterobacter asburiae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4951.43 Da
• Isoelectric Point: 4.1079

>AT213597 WP_000588713.1 NZ_CP081872:2360474-2360599 [Bacillus toyonensis]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEKLEEELKRK

Nucleotide

• Length: 126 bp

>AT213597 NZ_CP109697:c41225-40959 [Enterobacter asburiae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: K0FNW8

Antitoxin

• AlphaFold DB: J8KE32