Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 234210..234852 Replicon chromosome
Accession NZ_CP081872
Organism Bacillus toyonensis strain UTDF19-29B

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag K6T24_RS01330 Protein ID WP_000635963.1
Coordinates 234502..234852 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag K6T24_RS01325 Protein ID WP_000004570.1
Coordinates 234210..234497 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T24_RS01300 (K6T24_01295) 229538..230500 + 963 WP_098624155.1 UV DNA damage repair endonuclease UvsE -
K6T24_RS01305 (K6T24_01300) 230493..231065 - 573 WP_000908503.1 rhomboid family intramembrane serine protease -
K6T24_RS01310 (K6T24_01305) 231158..231517 + 360 WP_016107395.1 holo-ACP synthase -
K6T24_RS01315 (K6T24_01310) 231674..232624 + 951 WP_002059179.1 outer membrane lipoprotein carrier protein LolA -
K6T24_RS01320 (K6T24_01315) 232742..233911 + 1170 WP_000390621.1 alanine racemase -
K6T24_RS01325 (K6T24_01320) 234210..234497 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
K6T24_RS01330 (K6T24_01325) 234502..234852 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K6T24_RS01335 (K6T24_01330) 234921..237089 + 2169 WP_000426216.1 Tex family protein -
K6T24_RS01340 (K6T24_01335) 237147..237263 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
K6T24_RS01345 (K6T24_01340) 237471..237929 + 459 WP_000544526.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T213596 WP_000635963.1 NZ_CP081872:234502-234852 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T213596 NZ_CP109697:c27646-27359 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT213596 WP_000004570.1 NZ_CP081872:234210-234497 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT213596 NZ_CP109697:c27885-27646 [Enterobacter asburiae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References