Detailed information of TA system TA213595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 309801..310437
• Replicon: chromosome
• Accession: NZ_CP081870
• Organism: Rossellomorea marisflavi strain UTDF19-31A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K6T23_RS01665
• Protein ID: WP_048007230.1
• Coordinates: 310087..310437 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0J5SXN2
• Locus tag: K6T23_RS01660
• Protein ID: WP_048007231.1
• Coordinates: 309801..310082 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T23_RS01640 (K6T23_01650)	306094..306678	-	585	WP_056541591.1	rhomboid family intramembrane serine protease	-
K6T23_RS01645 (K6T23_01655)	306797..307147	+	351	WP_238284362.1	holo-ACP synthase	-
K6T23_RS01650 (K6T23_01660)	307346..308359	+	1014	WP_053427972.1	outer membrane lipoprotein carrier protein LolA	-
K6T23_RS01655 (K6T23_01665)	308486..309631	+	1146	WP_273546595.1	alanine racemase	-
K6T23_RS01660 (K6T23_01670)	309801..310082	+	282	WP_048007231.1	YlcI/YnfO family protein	Antitoxin
K6T23_RS01665 (K6T23_01675)	310087..310437	+	351	WP_048007230.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K6T23_RS01670 (K6T23_01680)	310790..311620	+	831	WP_056541597.1	RsbT co-antagonist protein RsbRA	-
K6T23_RS01675 (K6T23_01685)	311617..311973	+	357	WP_048007228.1	STAS domain-containing protein	-
K6T23_RS01680 (K6T23_01690)	311976..312377	+	402	WP_056541599.1	anti-sigma regulatory factor	-
K6T23_RS01685 (K6T23_01695)	312388..313398	+	1011	WP_056541601.1	PP2C family protein-serine/threonine phosphatase	-
K6T23_RS01690 (K6T23_01700)	313473..313805	+	333	WP_056541604.1	anti-sigma factor antagonist	-
K6T23_RS01695 (K6T23_01705)	313802..314272	+	471	WP_053427977.1	anti-sigma B factor RsbW	-
K6T23_RS01700 (K6T23_01710)	314250..315047	+	798	WP_053427978.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13007.02 Da
• Isoelectric Point: 5.1554

>T213595 WP_048007230.1 NZ_CP081870:310087-310437 [Rossellomorea marisflavi]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDSKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDDALQISVGLIQF

Nucleotide

• Length: 351 bp

>T213595 NZ_CP109695:c17871-17455 [Enterobacter asburiae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10789.24 Da
• Isoelectric Point: 6.4767

>AT213595 WP_048007231.1 NZ_CP081870:309801-310082 [Rossellomorea marisflavi]
VSESSATTEILIRLPQQLVTELDGFADQENVNRNEFIYRATKMYLRERKKRHLRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT213595 NZ_CP109695:c18098-17868 [Enterobacter asburiae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J5SXN2