Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5288765..5289390 Replicon chromosome
Accession NZ_CP081865
Organism Klebsiella pneumoniae strain K5880C

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6J95_RS25755 Protein ID WP_002882817.1
Coordinates 5288765..5289148 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6J95_RS25760 Protein ID WP_004150355.1
Coordinates 5289148..5289390 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J95_RS25740 (5286131) 5286131..5287033 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6J95_RS25745 (5287030) 5287030..5287665 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6J95_RS25750 (5287662) 5287662..5288591 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6J95_RS25755 (5288765) 5288765..5289148 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J95_RS25760 (5289148) 5289148..5289390 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6J95_RS25765 (5289595) 5289595..5290512 + 918 WP_021466676.1 alpha/beta hydrolase -
K6J95_RS25770 (5290526) 5290526..5291467 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6J95_RS25775 (5291512) 5291512..5291949 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6J95_RS25780 (5291946) 5291946..5292806 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6J95_RS25785 (5292800) 5292800..5293399 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213592 WP_002882817.1 NZ_CP081865:c5289148-5288765 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213592 NZ_CP109694:c4508064-4507777 [Enterobacter asburiae]
ATGCATAAATTAACACCCTACGATCCAGCCAATGCGCTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCCTT
AGAAACGGGCGACTCAGCGTATATTGCTAAAGCTCTGGGCGTCATCGCGCGAGCGAAAGGAATGTCGACCATTTCACAGC
AAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTAACAATCAAACACGCCGAAGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213592 WP_004150355.1 NZ_CP081865:c5289390-5289148 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213592 NZ_CP109694:c4508369-4508067 [Enterobacter asburiae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCAACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGTTTAGCGGGCGACATTAAACCCGTGGGCGAAGGTATAAGTGAGCTGAGGATCC
ACTATGGTCCGGGCTATAGACTCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATCCCAATGGCAGGAGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References