Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4693348..4694158 Replicon chromosome
Accession NZ_CP081865
Organism Klebsiella pneumoniae strain K5880C

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K6J95_RS22950 Protein ID WP_002887280.1
Coordinates 4693348..4693881 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6J95_RS22955 Protein ID WP_002887278.1
Coordinates 4693892..4694158 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J95_RS22945 (4692180) 4692180..4693301 + 1122 WP_009309849.1 cupin domain-containing protein -
K6J95_RS22950 (4693348) 4693348..4693881 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K6J95_RS22955 (4693892) 4693892..4694158 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6J95_RS22960 (4694261) 4694261..4695694 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
K6J95_RS22965 (4695684) 4695684..4696367 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
K6J95_RS22970 (4696540) 4696540..4697925 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
K6J95_RS22975 (4697943) 4697943..4698248 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T213589 WP_002887280.1 NZ_CP081865:c4693881-4693348 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213589 NZ_CP109694:c3390510-3390100 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGATTGCGGC
CTTTGTTTTGCTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213589 WP_002887278.1 NZ_CP081865:c4694158-4693892 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213589 NZ_CP109694:c3390757-3390491 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References